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Technology Advancement for Integrative Stem Cell Analyses #MMPMID24874188
Jeong Y; Choi J; Lee KH
Tissue Eng Part B Rev 2014[Dec]; 20 (6): 669-82 PMID24874188show ga
Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose?by introducing a concept of vertical and horizontal approach?that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment.
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Tissue+Eng+Part+B+Rev 2014 ; 20 (6): 669-82
