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2014 ; 20
(6
): 683-96
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Review of collagen I hydrogels for bioengineered tissue microenvironments:
characterization of mechanics, structure, and transport
#MMPMID24923709
Antoine EE
; Vlachos PP
; Rylander MN
Tissue Eng Part B Rev
2014[Dec]; 20
(6
): 683-96
PMID24923709
show ga
Type I collagen hydrogels have been used successfully as three-dimensional
substrates for cell culture and have shown promise as scaffolds for engineered
tissues and tumors. A critical step in the development of collagen hydrogels as
viable tissue mimics is quantitative characterization of hydrogel properties and
their correlation with fabrication parameters, which enables hydrogels to be
tuned to match specific tissues or fulfill engineering requirements. A
significant body of work has been devoted to characterization of collagen I
hydrogels; however, due to the breadth of materials and techniques used for
characterization, published data are often disjoint and hence their utility to
the community is reduced. This review aims to determine the parameter space
covered by existing data and identify key gaps in the literature so that future
characterization and use of collagen I hydrogels for research can be most
efficiently conducted. This review is divided into three sections: (1) relevant
fabrication parameters are introduced and several of the most popular methods of
controlling and regulating them are described, (2) hydrogel properties most
relevant for tissue engineering are presented and discussed along with their
characterization techniques, (3) the state of collagen I hydrogel
characterization is recapitulated and future directions are proposed. Ultimately,
this review can serve as a resource for selection of fabrication parameters and
material characterization methodologies in order to increase the usefulness of
future collagen-hydrogel-based characterization studies and tissue engineering
experiments.