Tissue myeloid cells in SIV-infected primates acquire viral DNA through
phagocytosis of infected T cells
#MMPMID25238099
Calantone N
; Wu F
; Klase Z
; Deleage C
; Perkins M
; Matsuda K
; Thompson EA
; Ortiz AM
; Vinton CL
; Ourmanov I
; Loré K
; Douek DC
; Estes JD
; Hirsch VM
; Brenchley JM
Immunity
2014[Sep]; 41
(3
): 493-502
PMID25238099
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The viral accessory protein Vpx, expressed by certain simian and human
immunodeficiency viruses (SIVs and HIVs), is thought to improve viral infectivity
of myeloid cells. We infected 35 Asian macaques and African green monkeys with
viruses that do or do not express Vpx and examined viral targeting of cells in
vivo. While lack of Vpx expression affected viral dynamics in vivo, with
decreased viral loads and infection of CD4? T cells, Vpx expression had no
detectable effect on infectivity of myeloid cells. Moreover, viral DNA was
observed only within myeloid cells in tissues not massively depleted of CD4? T
cells. Myeloid cells containing viral DNA also showed evidence of T cell
phagocytosis in vivo, suggesting that their viral DNA may be attributed to
phagocytosis of SIV-infected T cells. These data suggest that myeloid cells are
not a major source of SIV in vivo, irrespective of Vpx expression.
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