Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Shock 2014 ; 42 (6): 485-98 Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Lymph is not a Plasma Ultrafiltrate: A Proteomic Analysis of Injured Patients #MMPMID25243428
Dzieciatkowska M; D'Alessandro A; Moore EE; Wohlauer M; Banerjee A; Silliman CC; Hansen KC
Shock 2014[Dec]; 42 (6): 485-98 PMID25243428show ga
Studies on animal models have documented a role for the water soluble protein fraction of mesenteric lymph as a conduit from hemorrhagic shock to acute lung injury and post-injury multiple organ failure. We hypothesize that mesenteric lymph is not an ultrafiltrate of plasma and contains specific protein mediators that may predispose patients to ALI/MOF. Mesenteric lymph and plasma were collected from critically ill or injured patients and from nine patients with lymphatic injuries, during semi-elective spine reconstruction, or immediately before organ donation. Proteomic analyses were performed through immuno-affinity depletion of the 14 most abundant plasma proteins, and GeLC-MS analyses. Overall, 548 proteins were identified in the patients undergoing semi-elective surgery, of which 155 were uniquely present in the lymph. In addition, the post-shock plasma proteome was characterized by peculiar features, suggesting that only a partial overlap exists between the plasma and mesenteric lymph from trauma patients. Differential proteins between the matched plasma and mesenteric lymph from trauma patients could be related to, coagulopathy and hypercoagulability, cell lysis, pro-inflammatory responses and immune system activation, extracellular matrix remodeling, lymph-specific immunomodulation and vascular hypoactivity/neoangiogenesis, and energy/redox metabolic adaptation to trauma. In conclusion, the proteome of mesenteric lymph is biologically different (in qualitative and quantitative terms) than that of a mere plasma ultrafiltrate.