Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1152/ajpcell.00029.2014 http://scihub22266oqcxt.onion/10.1152/ajpcell.00029.2014 C4233262!4233262!25231106     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol+Cell+Physiol 2014 ; 307 (10): C920-7 Nephropedia Template TP {{tp|p=25231106|t=2014. Sphingosine kinase 1 (Sphk1) negatively regulates platelet activation and thrombus formation |pdf=|usr=}}{{25231106}} gab.com Text Sphingosine kinase 1 (Sphk1) negatively regulates platelet activation and thrombus formation JO: Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=25231106 #FOAvip Sphingosine 1-phosphate (S1P) is a powerful regulator of platelet formation. Enzymes generating S1P include sphingosine kinase 1. The present study thus explored the role of sphingosine kinase 1 in platelet formation and function. Activation-dependent platelet integrin ?IIb?3 activation and secretion of platelets lacking functional sphingosine kinase 1 (sphk1?/?) and of wild-type platelets (sphk1+/+) were determined utilizing flow cytometry and chronolume luciferin assay. Cytosolic Ca2+ activity ([Ca2+]i) and aggregation were measured using fura-2 fluorescence and aggregometry, respectively. In vitro platelet adhesion and thrombus formation were evaluated using a flow chamber with shear rates of 1,700 s?1. Activation-dependent increase of [Ca2+]i, degranulation (release of alpha and dense granules), integrin ?IIb?3 activation, and aggregation were all significantly increased in sphk1?/? platelets compared with sphk1+/+ platelets. Moreover, while platelet adhesion and thrombus formation under arterial shear rates were significantly augmented in Sphk1-deficient platelets, bleeding time and blood count were unaffected in sphk1?/? mice. In conclusion, sphingosine kinase 1 is a powerful negative regulator of platelet function counteracting degranulation, aggregation, and thrombus formation. Twit Text FOAVip Sphingosine kinase 1 (Sphk1) negatively regulates platelet activation and thrombus formation ????Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=25231106 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25231106 #FOAvip English Wikipedia <ref>{{Cite pmid|25231106}}</ref> Sphingosine kinase 1 (Sphk1) negatively regulates platelet activation and thrombus formation #MMPMID25231106 Münzer P; Schmid E; Walker B; Fotinos A; Chatterjee M; Rath D; Vogel S; Hoffmann SM; Metzger K; Seizer P; Geisler T; Gawaz M; Borst O; Lang F Am J Physiol Cell Physiol 2014[Nov]; 307 (10): C920-7 PMID25231106show ga Sphingosine 1-phosphate (S1P) is a powerful regulator of platelet formation. Enzymes generating S1P include sphingosine kinase 1. The present study thus explored the role of sphingosine kinase 1 in platelet formation and function. Activation-dependent platelet integrin ?IIb?3 activation and secretion of platelets lacking functional sphingosine kinase 1 (sphk1?/?) and of wild-type platelets (sphk1+/+) were determined utilizing flow cytometry and chronolume luciferin assay. Cytosolic Ca2+ activity ([Ca2+]i) and aggregation were measured using fura-2 fluorescence and aggregometry, respectively. In vitro platelet adhesion and thrombus formation were evaluated using a flow chamber with shear rates of 1,700 s?1. Activation-dependent increase of [Ca2+]i, degranulation (release of alpha and dense granules), integrin ?IIb?3 activation, and aggregation were all significantly increased in sphk1?/? platelets compared with sphk1+/+ platelets. Moreover, while platelet adhesion and thrombus formation under arterial shear rates were significantly augmented in Sphk1-deficient platelets, bleeding time and blood count were unaffected in sphk1?/? mice. In conclusion, sphingosine kinase 1 is a powerful negative regulator of platelet function counteracting degranulation, aggregation, and thrombus formation. äSphingosine kinase 1 (Sphk1) negatively regulates platelet activation (epmc).pdf DeepDyve Pubget Overpricing