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10.1007/s12013-014-9982-8 http://scihub22266oqcxt.onion/10.1007/s12013-014-9982-8 C4228983!4228983 !24839062     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24839062 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell+Biochem+Biophys 2014 ; 70 (2 ): 785-94 Nephropedia Template TP {{tp|p=24839062 |t=2014. Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development |pdf=|usr=}}{{24839062 }} gab.com Text Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development JO: Cell Biochem Biophys http://www.kidney.de/pget.php?&tw=1&pmid=24839062 #FOAvip We describe a method based on fluorescence-lifetime imaging microscopy (FLIM) to assess the fluidity of various membranes in neuronal cells at different stages of development [day 12 (E12) and day 16 (E16) of gestation]. For the FLIM measurements, we use the Laurdan probe which is commonly used to assess membrane water penetration in model and in biological membranes using spectral information. Using the FLIM approach, we build a fluidity scale based on calibration with model systems of different lipid compositions. In neuronal cells, we found a marked difference in fluidity between the internal membranes and the plasma membrane, being the plasma membrane the less fluid. However, we found no significant differences between the two cell groups, E12 and E16. Comparison with NIH3T3 cells shows that the plasma membranes of E12 and E16 cells are significantly more fluid than the plasma membrane of the cancer cells. Twit Text FOAVip Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development ????Cell Biochem Biophys http://www.kidney.de/pget.php?&tw=1&pmid=24839062 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24839062 #FOAvip English Wikipedia <ref>{{Cite pmid|24839062 }}</ref> Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development #MMPMID24839062 Bonaventura G ; Barcellona ML ; Golfetto O ; Nourse JL ; Flanagan LA ; Gratton E Cell Biochem Biophys 2014[Nov]; 70 (2 ): 785-94 PMID24839062 show ga We describe a method based on fluorescence-lifetime imaging microscopy (FLIM) to assess the fluidity of various membranes in neuronal cells at different stages of development [day 12 (E12) and day 16 (E16) of gestation]. For the FLIM measurements, we use the Laurdan probe which is commonly used to assess membrane water penetration in model and in biological membranes using spectral information. Using the FLIM approach, we build a fluidity scale based on calibration with model systems of different lipid compositions. In neuronal cells, we found a marked difference in fluidity between the internal membranes and the plasma membrane, being the plasma membrane the less fluid. However, we found no significant differences between the two cell groups, E12 and E16. Comparison with NIH3T3 cells shows that the plasma membranes of E12 and E16 cells are significantly more fluid than the plasma membrane of the cancer cells. |*Embryonic Development [MESH] |*Lipid Metabolism [MESH] |2-Naphthylamine/*analogs & derivatives/chemistry [MESH] |Animals [MESH] |Cell Membrane/*metabolism [MESH] |Cholesterol/metabolism [MESH] |Female [MESH] |Fluorescent Dyes/*chemistry [MESH] |Laurates/*chemistry [MESH] |Membrane Fluidity [MESH] |Mice [MESH] |Microscopy, Fluorescence/*methods [MESH] |NIH 3T3 Cells [MESH] |Neurons/*cytology [MESH] |Pregnancy [MESH]Laurdan monitors different lipids content in eukaryotic membrane durin(epmc).pdf DeepDyve Pubget Overpricing