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Morphological and cytochemical characterization of cells infiltrating mouse lungs
after influenza infection
#MMPMID711312
Wyde PR
; Peavy DL
; Cate TR
Infect Immun
1978[Jul]; 21
(1
): 140-6
PMID711312
show ga
To initiate evaluation of the cell-mediated immunological response to influenza
virus in a major site of disease, lung cells were obtained by transpleural lavage
from lungs of uninfected mice and from those infected 3 or 6 days previously with
5 50% mouse infectious doses (MID(50)) of avirulent (P3) or virulent (P9)
influenza A Hong Kong (H3N2) virus. The number of cells recovered by lavage was
dependent on the dose, time after inoculation, and the type of virus used for
inoculation. Although lavage pools were shown to contain peripheral blood
leukocytes, this contamination was shown to be consistently less than 5% of the
total leukocytes harvested. Among the ca. 0.75 x 10(6) lavage cells obtained from
each uninfected mouse, about 90% were macrophages or lymphocytes in approximately
equal proportion. T, B, and null (lyphocytes lacking theta or surface
immunoglobulin markers) lymphocytes averaged 23, 9, and 7% of cells in these
suspensions, respectively. After infection with either P3 or P9 virus, increased
numbers of activated macrophages and lymphoblasts were observed. The major change
during P3 infection was an increase in absolute numbers of null lymphocytes. In
contrast, during P9 infection, T and B lymphocytes and macrophages progressively
increased in absolute numbers while null cells decreased. These data suggest that
cell-mediated immunological responses to influenza virus occur in the lung during
infection, but that the responses to virulent and avirulent variants may differ
both qualitatively and quantitatively.
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