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10.1021/jp5025119 http://scihub22266oqcxt.onion/10.1021/jp5025119 C4216197!4216197!24950158     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Phys+Chem+B 2014 ; 118 (30): 8945-55 Nephropedia Template TP {{tp|p=24950158|t=2014. Water Behavior in Bacterial Spores by Deuterium NMR Spectroscopy |pdf=|usr=}}{{24950158}} gab.com Text Water Behavior in Bacterial Spores by Deuterium NMR Spectroscopy JO: J Phys Chem B http://www.kidney.de/pget.php?&tw=1&pmid=24950158 #FOAvip Dormant bacterial spores are able to survive long periods of time without nutrients, withstand harsh environmental conditions, and germinate into metabolically active bacteria when conditions are favorable. Numerous factors influence this hardiness, including the spore structure and the presence of compounds to protect DNA from damage. It is known that the water content of the spore core plays a role in resistance to degradation, but the exact state of water inside the core is a subject of discussion. Two main theories present themselves: either the water in the spore core is mostly immobile and the core and its components are in a glassy state, or the core is a gel with mobile water around components which themselves have limited mobility. Using deuterium solid-state NMR experiments, we examine the nature of the water in the spore core. Our data show the presence of unbound water, bound water, and deuterated biomolecules that also contain labile deuterons. Deuterium?hydrogen exchange experiments show that most of these deuterons are inaccessible by external water. We believe that these unreachable deuterons are in a chemical bonding state that prevents exchange. Variable-temperature NMR results suggest that the spore core is more rigid than would be expected for a gel-like state. However, our rigid core interpretation may only apply to dried spores whereas a gel core may exist in aqueous suspension. Nonetheless, the gel core, if present, is inaccessible to external water. Twit Text FOAVip Water Behavior in Bacterial Spores by Deuterium NMR Spectroscopy ????J Phys Chem B http://www.kidney.de/pget.php?&tw=1&pmid=24950158 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24950158 #FOAvip English Wikipedia <ref>{{Cite pmid|24950158}}</ref> Water Behavior in Bacterial Spores by Deuterium NMR Spectroscopy #MMPMID24950158 Friedline A; Zachariah MM; Johnson K; Thomas KJ; Middaugh AN; Garimella R; Powell D; Vaishampayan PA; Rice CV J Phys Chem B 2014[Jul]; 118 (30): 8945-55 PMID24950158show ga Dormant bacterial spores are able to survive long periods of time without nutrients, withstand harsh environmental conditions, and germinate into metabolically active bacteria when conditions are favorable. Numerous factors influence this hardiness, including the spore structure and the presence of compounds to protect DNA from damage. It is known that the water content of the spore core plays a role in resistance to degradation, but the exact state of water inside the core is a subject of discussion. Two main theories present themselves: either the water in the spore core is mostly immobile and the core and its components are in a glassy state, or the core is a gel with mobile water around components which themselves have limited mobility. Using deuterium solid-state NMR experiments, we examine the nature of the water in the spore core. Our data show the presence of unbound water, bound water, and deuterated biomolecules that also contain labile deuterons. Deuterium?hydrogen exchange experiments show that most of these deuterons are inaccessible by external water. We believe that these unreachable deuterons are in a chemical bonding state that prevents exchange. Variable-temperature NMR results suggest that the spore core is more rigid than would be expected for a gel-like state. However, our rigid core interpretation may only apply to dried spores whereas a gel core may exist in aqueous suspension. Nonetheless, the gel core, if present, is inaccessible to external water. äWater Behavior in Bacterial Spores by Deuterium NMR Spectroscopy (epmc).pdf DeepDyve Pubget Overpricing