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2014 ; 5
(2
): e28425
Nephropedia Template TP
gab.com Text
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English Wikipedia
Advanced fluorescence microscopy methods for the real-time study of transcription
and chromatin dynamics
#MMPMID25764219
Annibale P
; Gratton E
Transcription
2014[]; 5
(2
): e28425
PMID25764219
show ga
In this contribution we provide an overview of the recent advances allowed by the
use of fluorescence microscopy methods in the study of transcriptional processes
and their interplay with the chromatin architecture in living cells. Although the
use of fluorophores to label nucleic acids dates back at least to about half a
century ago, (1) two recent breakthroughs have effectively opened the way to use
fluorescence routinely for specific and quantitative probing of chromatin
organization and transcriptional activity in living cells: namely, the
possibility of labeling first the chromatin loci and then the mRNA synthesized
from a gene using fluorescent proteins. In this contribution we focus on methods
that can probe rapid dynamic processes by analyzing fast fluorescence
fluctuations.