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2014 ; 95
(5
): 321-9
Nephropedia Template TP
gab.com Text
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English Wikipedia
Bone marrow cells migrate to the heart and skeletal muscle and participate in
tissue repair after Trypanosoma cruzi infection in mice
#MMPMID24976301
Souza BS
; Azevedo CM
; d Lima RS
; Kaneto CM
; Vasconcelos JF
; Guimarães ET
; dos Santos RR
; Soares MB
Int J Exp Pathol
2014[Oct]; 95
(5
): 321-9
PMID24976301
show ga
Infection by Trypanosoma cruzi, the aetiological agent of Chagas disease, causes
an intense inflammatory reaction in several tissues, including the myocardium. We
have previously shown that transplantation of bone marrow cells (BMC) ameliorates
the myocarditis in a mouse model of chronic Chagas disease. We investigated the
participation of BMC in lesion repair in the heart and skeletal muscle, caused by
T. cruzi infection in mice. Infection with a myotropic T. cruzi strain induced an
increase in the percentage of stem cells and monocytes in the peripheral blood,
as well as in gene expression of chemokines SDF-1, MCP1, 2, and 3 in the heart
and skeletal muscle. To investigate the fate of BMC within the damaged tissue,
chimeric mice were generated by syngeneic transplantation of green fluorescent
protein (GFP(+) ) BMC into lethally irradiated mice and infected with Trypanosoma
cruzi. Migration of GFP(+) BMC to the heart and skeletal muscle was observed
during and after the acute phase of infection. GFP(+) cardiomyocytes and
endothelial cells were present in heart sections of chimeric chagasic mice.
GFP(+) myofibres were observed in the skeletal muscle of chimeric mice at
different time points following infection. In conclusion, BMC migrate and
contribute to the formation of new resident cells in the heart and skeletal
muscle, which can be detected both during the acute and the chronic phase of
infection. These findings reinforce the role of BMC in tissue regeneration.