Cell-Mediated Assembly of Phototherapeutics #MMPMID25154925
Smith WJ; Oien NP; Hughes RM; Marvin CM; Rodgers ZL; Lee J; Lawrence DS
Angew Chem Int Ed Engl 2014[Oct]; 53 (41): 10945-8 PMID25154925show ga
Light-activatable drugs offer the promise of controlled release with exquisite temporal and spatial resolution. However, light sensitive pro-drugs are typically converted to their active forms using short wavelengths, which display poor tissue penetrance. We report herein erythrocyte-mediated assembly of long wavelength-sensitive phototherapeutics. The activating wavelength of the constructs is readily pre-assigned by using fluorophores with the desired ?ex. Drug release from the erythrocyte carrier was confirmed by standard analytical tools and by the expected biological consequences of the liberated drugs in cell culture: methotrexate, binding to intracellular dihydrofolate reductase; colchicine, inhibition of microtubule polymerization; dexamethasone, induced nuclear migration of the glucocorticoid receptor.