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2014 ; 10
(7
): 1338-40
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Atg1 kinase organizes autophagosome formation by phosphorylating Atg9
#MMPMID24905091
Papinski D
; Kraft C
Autophagy
2014[Jul]; 10
(7
): 1338-40
PMID24905091
show ga
The conserved Ser/Thr kinase Atg1/ULK1 plays a crucial role in the regulation of
autophagy. However, only very few Atg1 targets have been identified, impeding
elucidation of the mechanisms by which Atg1 regulates autophagy. In our study, we
determined the Saccharomyces cerevisiae Atg1 consensus phosphorylation sequence
using a peptide array-based approach. Among proteins containing this sequence we
identified Atg9, another essential component of the autophagic machinery. We
showed that phosphorylation of Atg9 by Atg1 is required for phagophore
elongation, shedding light on the mechanism by which Atg1 regulates early steps
of autophagy.