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10.1124/dmd.114.060145 http://scihub22266oqcxt.onion/10.1124/dmd.114.060145 C4201134!4201134 !25161167     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25161167 &cmd=llinks): Failed to open stream: HTTP request failed! 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Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells |pdf=|usr=}}{{25161167 }} gab.com Text Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells JO: Drug Metab Dispos http://www.kidney.de/pget.php?&tw=1&pmid=25161167 #FOAvip Noncoding microRNAs (miRNAs or miRs) have been revealed as critical epigenetic factors in the regulation of various cellular processes, including drug metabolism and disposition. However, research on miRNA functions is limited to the use of synthetic RNA and recombinant DNA agents. Herein, we show that novel pre-miRNA-27b (miR-27b) agents can be biosynthesized in Escherichia coli using recombinant RNA technology, and recombinant transfer RNA (tRNA)/mir-27b chimera was readily purified to a high degree of homogeneity (>95%) using anion-exchange fast protein liquid chromatography. The tRNA-fusion miR-27b was revealed to be processed to mature miRNA miR-27b in human carcinoma LS-180 cells in a dose- and time-dependent manner. Moreover, recombinant tRNA/miR-27b agents were biologically active in reducing the mRNA and protein expression levels of cytochrome P450 3A4 (CYP3A4), which consequently led to lower midazolam 1'-hydroxylase activity. These findings demonstrate that pre-miRNA agents can be produced by recombinant RNA technology for functional studies. Twit Text FOAVip Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells ????Drug Metab Dispos http://www.kidney.de/pget.php?&tw=1&pmid=25161167 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25161167 #FOAvip English Wikipedia <ref>{{Cite pmid|25161167 }}</ref> Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells #MMPMID25161167 Li MM ; Wang WP ; Wu WJ ; Huang M ; Yu AM Drug Metab Dispos 2014[Nov]; 42 (11 ): 1791-5 PMID25161167 show ga Noncoding microRNAs (miRNAs or miRs) have been revealed as critical epigenetic factors in the regulation of various cellular processes, including drug metabolism and disposition. However, research on miRNA functions is limited to the use of synthetic RNA and recombinant DNA agents. Herein, we show that novel pre-miRNA-27b (miR-27b) agents can be biosynthesized in Escherichia coli using recombinant RNA technology, and recombinant transfer RNA (tRNA)/mir-27b chimera was readily purified to a high degree of homogeneity (>95%) using anion-exchange fast protein liquid chromatography. The tRNA-fusion miR-27b was revealed to be processed to mature miRNA miR-27b in human carcinoma LS-180 cells in a dose- and time-dependent manner. Moreover, recombinant tRNA/miR-27b agents were biologically active in reducing the mRNA and protein expression levels of cytochrome P450 3A4 (CYP3A4), which consequently led to lower midazolam 1'-hydroxylase activity. These findings demonstrate that pre-miRNA agents can be produced by recombinant RNA technology for functional studies. |*Recombination, Genetic [MESH] |Base Sequence [MESH] |Cell Line, Tumor [MESH] |Chromatography, Ion Exchange [MESH] |DNA Primers [MESH] |Escherichia coli/*genetics [MESH] |Humans [MESH] |MicroRNAs/biosynthesis/genetics/*pharmacology [MESH]Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichi(epmc).pdf DeepDyve Pubget Overpricing