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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Arthritis+Rheumatol
2014 ; 66
(10
): 2793-2803
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Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase
magnesium-dependent 1A autoantibodies in ankylosing spondylitis
#MMPMID24980965
Kim YG
; Sohn DH
; Zhao X
; Sokolove J
; Lindstrom TM
; Yoo B
; Lee CK
; Reveille JD
; Taurog JD
; Robinson WH
Arthritis Rheumatol
2014[Oct]; 66
(10
): 2793-2803
PMID24980965
show ga
OBJECTIVE: Although ankylosing spondylitis (AS) is driven by immune-mediated
processes, little is known about the presence and role of autoantibodies in this
disease. This study was undertaken to investigate whether autoantibodies occur in
and are involved in AS. METHODS: We performed human protein microarray analysis
of sera derived from patients with AS or other autoimmune disorders to identify
autoantibodies associated specifically with AS, and identified autoantibody
targeting of protein phosphatase magnesium-dependent 1A (PPM1A) in AS. We
performed enzyme-linked immunosorbent assay (ELISA) analysis of sera from 2
independent AS cohorts to confirm autoantibody targeting of PPM1A, and to assess
associations between levels of anti-PPM1A antibodies and AS disease severity or
response to anti-tumor necrosis factor (anti-TNF) therapy (as measured by Bath AS
Disease Activity Index [BASDAI] score). Levels of anti-PPM1A antibodies were also
evaluated in sera from rats transgenic for HLA-B27 and human ?2 -microglobulin.
The expression of PPM1A was assessed by immunohistochemistry in synovial tissue
samples from patients with AS, rheumatoid arthritis, or osteoarthritis. The role
of PPM1A in osteoblast differentiation was investigated by gene knockdown and
overexpression. RESULTS: AS was associated with autoantibody targeting of PPM1A,
and levels of anti-PPM1A autoantibodies were significantly higher in patients
with more advanced sacroiliitis and correlated positively with BASDAI score after
treatment with anti-TNF agents. The levels of anti-PPM1A autoantibodies were also
higher in the sera of transgenic rats that are prone to develop spondyloarthritis
than in those that are not. PPM1A was expressed in AS synovial tissue, and PPM1A
overexpression promoted osteoblast differentiation, whereas PPM1A knockdown
suppressed it. CONCLUSION: Anti-PPM1A autoantibodies are present in AS, and our
findings suggest that PPM1A may contribute to the pathogenic bone ankylosis
characteristic of AS.