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10.1016/j.bcmd.2014.06.002 http://scihub22266oqcxt.onion/10.1016/j.bcmd.2014.06.002 C4198503!4198503!24998898     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Blood+Cells+Mol+Dis 2014 ; 53 (4): 231-40 Nephropedia Template TP {{tp|p=24998898|t=2014. A Chemical Screen Identifies Small Molecules that Regulate Hepcidin Expression |pdf=|usr=}}{{24998898}} gab.com Text A Chemical Screen Identifies Small Molecules that Regulate Hepcidin Expression JO: Blood Cells Mol Dis http://www.kidney.de/pget.php?&tw=1&pmid=24998898 #FOAvip Hepcidin, a peptide hormone produced in the liver, decreases intestinal iron absorption and macrophage iron release via effects on ferroportin. Bone morphogenic protein and Stat3 signaling regulate Hepcidin's transcription. Hepcidin is a potential drug target for patients with iron overload syndromes because its levels are inappropriately low in these individuals. To generate a tool for identifying small molecules that modulate Hepcidin expression, we stably transfected human hepatocytes (HepG2) cells with a reporter construct containing 2.7 kilobases of the human Hepcidin promoter upstream of a firefly reporter gene. We used high throughput methods to screen 10,169 chemicals in duplicate for their effect on Hepcidin expression and cell viability. Regulators were identified as chemicals that caused a change >3 standard deviations above or >1.5 standard deviations below the mean of the other chemicals (z-score >3 or <-1.5), while not adversely affecting cell viability, quantified by fluorescence assay. Following validation assays, we identified 16 chemicals in a broad range of functional classes that promote Hepcidin expression. All of the chemicals identified increased expression of bone morphogenic protein-dependent and/or Stat3-dependent genes, however none of them strongly increased phosphorylation of Smad1,5,8 or Stat3. Twit Text FOAVip A Chemical Screen Identifies Small Molecules that Regulate Hepcidin Expression ????Blood Cells Mol Dis http://www.kidney.de/pget.php?&tw=1&pmid=24998898 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24998898 #FOAvip English Wikipedia <ref>{{Cite pmid|24998898}}</ref> A Chemical Screen Identifies Small Molecules that Regulate Hepcidin Expression #MMPMID24998898 Gaun V; Patchen B; Volovetz J; Zhen AW; Andreev A; Pollastri MP; Fraenkel PG Blood Cells Mol Dis 2014[Dec]; 53 (4): 231-40 PMID24998898show ga Hepcidin, a peptide hormone produced in the liver, decreases intestinal iron absorption and macrophage iron release via effects on ferroportin. Bone morphogenic protein and Stat3 signaling regulate Hepcidin's transcription. Hepcidin is a potential drug target for patients with iron overload syndromes because its levels are inappropriately low in these individuals. To generate a tool for identifying small molecules that modulate Hepcidin expression, we stably transfected human hepatocytes (HepG2) cells with a reporter construct containing 2.7 kilobases of the human Hepcidin promoter upstream of a firefly reporter gene. We used high throughput methods to screen 10,169 chemicals in duplicate for their effect on Hepcidin expression and cell viability. Regulators were identified as chemicals that caused a change >3 standard deviations above or >1.5 standard deviations below the mean of the other chemicals (z-score >3 or <-1.5), while not adversely affecting cell viability, quantified by fluorescence assay. Following validation assays, we identified 16 chemicals in a broad range of functional classes that promote Hepcidin expression. All of the chemicals identified increased expression of bone morphogenic protein-dependent and/or Stat3-dependent genes, however none of them strongly increased phosphorylation of Smad1,5,8 or Stat3. äA Chemical Screen Identifies Small Molecules that Regulate Hepcidin Ex(epmc).pdf DeepDyve Pubget Overpricing