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2014 ; 15
(6
): 670-7
Nephropedia Template TP
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A novel import route for an N-anchor mitochondrial outer membrane protein aided
by the TIM23 complex
#MMPMID24781694
Song J
; Tamura Y
; Yoshihisa T
; Endo T
EMBO Rep
2014[Jun]; 15
(6
): 670-7
PMID24781694
show ga
The membrane topology of Om45 in the yeast mitochondrial outer membrane (OM) is
under debate. Here, we confirm that Om45 is anchored to the OM from the
intermembrane space (IMS) by its N-terminal hydrophobic segment. We show that
import of Om45 requires the presequence receptors, Tom20 and Tom22, and the
import channel of Tom40. Unlike any of the known OM proteins, Om45 import
requires the TIM23 complex in the inner membrane, a translocator for
presequence-containing proteins, and the membrane potential (??). Therefore, Om45
is anchored to the OM via the IMS by a novel import pathway involving the TIM23
complex.