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10.1038/ncomms5729 http://scihub22266oqcxt.onion/10.1038/ncomms5729 C4197411!4197411!25311543     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2014 ; 5 (ä): 4729 Nephropedia Template TP {{tp|p=25311543|t=2014. Highly modular bow-tie gene circuits with programmable dynamic behavior |pdf=|usr=}}{{25311543}} gab.com Text Highly modular bow-tie gene circuits with programmable dynamic behavior JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=25311543 #FOAvip Synthetic gene circuits often require extensive mutual optimization of their components for successful operation, while modular and programmable design platforms are rare. A possible solution lies in the ?bow-tie? architecture, which stipulates a focal component - a ?knot? - uncoupling circuits? inputs and outputs, simplifying component swapping, and introducing additional layer of control. Here we construct, in cultured human cells, synthetic bow-tie circuits that transduce microRNA inputs into protein outputs with independently programmable logical and dynamic behavior. The latter is adjusted via two different knot configurations: a transcriptional activator causing the outputs to track input changes reversibly, and a recombinase-based cascade, converting transient inputs into permanent actuation. We characterize the circuits in HEK293 cells, confirming their modularity and scalability, and validate them using endogenous microRNA inputs in additional cell lines. This platform can be used for biotechnological and biomedical applications in vitro, in vivo, and potentially in human therapy. Twit Text FOAVip Highly modular bow-tie gene circuits with programmable dynamic behavior ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=25311543 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25311543 #FOAvip English Wikipedia <ref>{{Cite pmid|25311543}}</ref> Highly modular bow-tie gene circuits with programmable dynamic behavior #MMPMID25311543 Prochazka L; Angelici B; Häfliger B; Benenson Y Nat Commun 2014[]; 5 (ä): 4729 PMID25311543show ga Synthetic gene circuits often require extensive mutual optimization of their components for successful operation, while modular and programmable design platforms are rare. A possible solution lies in the ?bow-tie? architecture, which stipulates a focal component - a ?knot? - uncoupling circuits? inputs and outputs, simplifying component swapping, and introducing additional layer of control. Here we construct, in cultured human cells, synthetic bow-tie circuits that transduce microRNA inputs into protein outputs with independently programmable logical and dynamic behavior. The latter is adjusted via two different knot configurations: a transcriptional activator causing the outputs to track input changes reversibly, and a recombinase-based cascade, converting transient inputs into permanent actuation. We characterize the circuits in HEK293 cells, confirming their modularity and scalability, and validate them using endogenous microRNA inputs in additional cell lines. This platform can be used for biotechnological and biomedical applications in vitro, in vivo, and potentially in human therapy. äHighly modular bow-tie gene circuits with programmable dynamic behavio(epmc).pdf DeepDyve Pubget Overpricing