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10.1002/anie.201404198 http://scihub22266oqcxt.onion/10.1002/anie.201404198 C4196877!4196877!25156888     free     free     free       Angew+Chem+Int+Ed+Engl 2014 ; 53 (41): 10904-7 Nephropedia Template TP {{tp|p=25156888|t=2014. Optogenetic Engineering: Light-Directed Cell Motility |pdf=|usr=}}{{25156888}} gab.com Text Optogenetic Engineering: Light-Directed Cell Motility JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25156888 #FOAvip Genetically encoded, light activatable proteins furnish the means to probe biochemical pathways at specific sub-cellular locations with exquisite temporal control. However, engineering these systems to provide a dramatic jump in localized activity while retaining a low dark-state background remains a significant challenge. We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination. We demonstrate that the use of a partially impaired mutant of cofilin is critical for maintaining low background activity in the dark. We also show that light-directed recruitment of the reduced activity cofilin mutants to the cytoskeleton is sufficient to induce F-actin remodeling, formation of filopodia, and directed cell motility. Twit Text FOAVip Optogenetic Engineering: Light-Directed Cell Motility ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25156888 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25156888 #FOAvip English Wikipedia <ref>{{Cite pmid|25156888}}</ref> Optogenetic Engineering: Light-Directed Cell Motility #MMPMID25156888 Hughes RM; Lawrence DS Angew Chem Int Ed Engl 2014[Oct]; 53 (41): 10904-7 PMID25156888show ga Genetically encoded, light activatable proteins furnish the means to probe biochemical pathways at specific sub-cellular locations with exquisite temporal control. However, engineering these systems to provide a dramatic jump in localized activity while retaining a low dark-state background remains a significant challenge. We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination. We demonstrate that the use of a partially impaired mutant of cofilin is critical for maintaining low background activity in the dark. We also show that light-directed recruitment of the reduced activity cofilin mutants to the cytoskeleton is sufficient to induce F-actin remodeling, formation of filopodia, and directed cell motility. äOptogenetic Engineering: Light-Directed Cell Motility (epmc).pdf DeepDyve Pubget Overpricing