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10.1093/aob/mcu042 http://scihub22266oqcxt.onion/10.1093/aob/mcu042 C4195548!4195548!24723448     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Ann+Bot 2014 ; 114 (6): 1189-201 Nephropedia Template TP {{tp|p=24723448|t=2014. New views of tapetum ultrastructure and pollen exine development in Arabidopsis thaliana |pdf=|usr=}}{{24723448}} gab.com Text New views of tapetum ultrastructure and pollen exine development in Arabidopsis thaliana JO: Ann Bot http://www.kidney.de/pget.php?&tw=1&pmid=24723448 #FOAvip Background and Aims: The Arabidopsis thaliana pollen cell wall is a complex structure consisting of an outer sporopollenin framework and lipid-rich coat, as well as an inner cellulosic wall. Although mutant analysis has been a useful tool to study pollen cell walls, the ultrastructure of the arabidopsis anther has proved to be challenging to preserve for electron microscopy. Methods: In this work, high-pressure freezing/freeze substitution and transmission electron microscopy were used to examine the sequence of developmental events in the anther that lead to sporopollenin deposition to form the exine and the dramatic differentiation and death of the tapetum, which produces the pollen coat. Key Results: Cryo-fixation revealed a new view of the interplay between sporophytic anther tissues and gametophytic microspores over the course of pollen development, especially with respect to the intact microspore/pollen wall and the continuous tapetum epithelium. These data reveal the ultrastructure of tapetosomes and elaioplasts, highly specialized tapetum organelles that accumulate pollen coat components. The tapetum and middle layer of the anther also remain intact into the tricellular pollen and late uninucleate microspore stages, respectively. Conclusions: This high-quality structural information, interpreted in the context of recent functional studies, provides the groundwork for future mutant studies where tapetum and microspore ultrastructure is assessed. Twit Text FOAVip New views of tapetum ultrastructure and pollen exine development in Arabidopsis thaliana ????Ann Bot http://www.kidney.de/pget.php?&tw=1&pmid=24723448 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24723448 #FOAvip English Wikipedia <ref>{{Cite pmid|24723448}}</ref> New views of tapetum ultrastructure and pollen exine development in Arabidopsis thaliana #MMPMID24723448 Quilichini TD; Douglas CJ; Samuels AL Ann Bot 2014[Oct]; 114 (6): 1189-201 PMID24723448show ga Background and Aims: The Arabidopsis thaliana pollen cell wall is a complex structure consisting of an outer sporopollenin framework and lipid-rich coat, as well as an inner cellulosic wall. Although mutant analysis has been a useful tool to study pollen cell walls, the ultrastructure of the arabidopsis anther has proved to be challenging to preserve for electron microscopy. Methods: In this work, high-pressure freezing/freeze substitution and transmission electron microscopy were used to examine the sequence of developmental events in the anther that lead to sporopollenin deposition to form the exine and the dramatic differentiation and death of the tapetum, which produces the pollen coat. Key Results: Cryo-fixation revealed a new view of the interplay between sporophytic anther tissues and gametophytic microspores over the course of pollen development, especially with respect to the intact microspore/pollen wall and the continuous tapetum epithelium. These data reveal the ultrastructure of tapetosomes and elaioplasts, highly specialized tapetum organelles that accumulate pollen coat components. The tapetum and middle layer of the anther also remain intact into the tricellular pollen and late uninucleate microspore stages, respectively. Conclusions: This high-quality structural information, interpreted in the context of recent functional studies, provides the groundwork for future mutant studies where tapetum and microspore ultrastructure is assessed. äNew views of tapetum ultrastructure and pollen exine development in Ar(epmc).pdf DeepDyve Pubget Overpricing