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2014 ; 8
(5
): 056501
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Towards plug and play filling of microfluidic devices by utilizing networks of
capillary stop valves
#MMPMID25332747
Hagmeyer B
; Zechnall F
; Stelzle M
Biomicrofluidics
2014[Sep]; 8
(5
): 056501
PMID25332747
show ga
Robust bubble-free priming of complex microfluidic chips represents a critical,
yet often unmet prerequisite to enable their practical and widespread
application. Towards this end, the usage of a network of capillary stop valves as
a generic design feature is proposed. Design principles, numerical simulations,
and their application in the development of a microfluidic cell culture device
are presented. This chip comprises eight parallel chambers for the assembly and
cultivation of human hepatocytes and endothelial cells. The inlet channel divides
into cell chambers, after which the flows are reunited to a single chip outlet.
Dimensions and geometry of channels and cell chambers are designed to yield
capillary burst pressures sequentially increasing towards the chip outlet. Thus,
progress of liquid flow through the device is predefined by design and enclosure
of air bubbles inside the microfluidic structures is efficiently avoided.
Capillary stop valves were designed using numerical simulations. Devices were
fabricated in cyclic olefin polymer. Pressure during filling was determined
experimentally and is in good agreement with data obtained from simulation.