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10.1038/nsmb.2892 http://scihub22266oqcxt.onion/10.1038/nsmb.2892 C4189996!4189996!25240799     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Struct+Mol+Biol 2014 ; 21 (10): 901-10 Nephropedia Template TP {{tp|p=25240799|t=2014. Rbfox3 Controls the Biogenesis of a Subset of MicroRNAs |pdf=|usr=}}{{25240799}} gab.com Text Rbfox3 Controls the Biogenesis of a Subset of MicroRNAs JO: Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25240799 #FOAvip RNA-binding proteins (RBPs) regulate numerous aspects of gene expression, thus identification of endogenous targets of RBPs is important for understanding their functions in cells. Here we identified transcriptome-wide targets of Rbfox3 in neuronally differentiated P19 cells and mouse brain using Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP). Although Rbfox3 is known to regulate pre-mRNA splicing through binding to the UGCAUG motif, PAR-CLIP analysis revealed diverse Rbfox3 targets including primary-microRNAs (pri-miRNAs) which lack the UGCAUG motif. Induced expression and depletion of Rbfox3 led to changes in the expression levels of a subset of PAR-CLIP-detected miRNAs. In vitro analyses revealed that Rbfox3 functions as a positive and a negative regulator at the stage of pri-miRNA processing to precursor-miRNA. Rbfox3 binds directly to pri-miRNAs and regulates the recruitment of the microprocessor complex to pri-miRNAs. Our study proposes a novel function for Rbfox3 in miRNA biogenesis. Twit Text FOAVip Rbfox3 Controls the Biogenesis of a Subset of MicroRNAs ????Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25240799 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25240799 #FOAvip English Wikipedia <ref>{{Cite pmid|25240799}}</ref> Rbfox3 Controls the Biogenesis of a Subset of MicroRNAs #MMPMID25240799 Kim KK; Yang Y; Zhu J; Adelstein RS; Kawamoto S Nat Struct Mol Biol 2014[Oct]; 21 (10): 901-10 PMID25240799show ga RNA-binding proteins (RBPs) regulate numerous aspects of gene expression, thus identification of endogenous targets of RBPs is important for understanding their functions in cells. Here we identified transcriptome-wide targets of Rbfox3 in neuronally differentiated P19 cells and mouse brain using Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP). Although Rbfox3 is known to regulate pre-mRNA splicing through binding to the UGCAUG motif, PAR-CLIP analysis revealed diverse Rbfox3 targets including primary-microRNAs (pri-miRNAs) which lack the UGCAUG motif. Induced expression and depletion of Rbfox3 led to changes in the expression levels of a subset of PAR-CLIP-detected miRNAs. In vitro analyses revealed that Rbfox3 functions as a positive and a negative regulator at the stage of pri-miRNA processing to precursor-miRNA. Rbfox3 binds directly to pri-miRNAs and regulates the recruitment of the microprocessor complex to pri-miRNAs. Our study proposes a novel function for Rbfox3 in miRNA biogenesis. äRbfox3 Controls the Biogenesis of a Subset of MicroRNAs (epmc).pdf DeepDyve Pubget Overpricing