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2014 ; 85
(10
): 103101
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A 32-channel photon counting module with embedded auto/cross-correlators for
real-time parallel fluorescence correlation spectroscopy
#MMPMID25362365
Gong S
; Labanca I
; Rech I
; Ghioni M
Rev Sci Instrum
2014[Oct]; 85
(10
): 103101
PMID25362365
show ga
Fluorescence correlation spectroscopy (FCS) is a well-established technique to
study binding interactions or the diffusion of fluorescently labeled biomolecules
in vitro and in vivo. Fast FCS experiments require parallel data acquisition and
analysis which can be achieved by exploiting a multi-channel Single Photon
Avalanche Diode (SPAD) array and a corresponding multi-input correlator. This
paper reports a 32-channel FPGA based correlator able to perform 32
auto/cross-correlations simultaneously over a lag-time ranging from 10 ns up to
150 ms. The correlator is included in a 32 × 1 SPAD array module, providing a
compact and flexible instrument for high throughput FCS experiments. However,
some inherent features of SPAD arrays, namely afterpulsing and optical crosstalk
effects, may introduce distortions in the measurement of auto- and
cross-correlation functions. We investigated these limitations to assess their
impact on the module and evaluate possible workarounds.