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2014 ; 108
(ä): 21.28.1-21.28.16
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Genome-Wide Analysis of Nucleosome Positions, Occupancy, and Accessibility in
Yeast: Nucleosome Mapping, High-Resolution Histone ChIP, and NCAM
#MMPMID25271716
Rodriguez J
; McKnight JN
; Tsukiyama T
Curr Protoc Mol Biol
2014[Oct]; 108
(ä): 21.28.1-21.28.16
PMID25271716
show ga
Because histones bind DNA very tightly, the location on DNA and the level of
occupancy of a given DNA sequence by nucleosomes can profoundly affect
accessibility of non-histone proteins to chromatin, affecting virtually all
DNA-dependent processes, such as transcription, DNA repair, DNA replication and
recombination. Therefore, it is often necessary to determine positions and
occupancy of nucleosomes to understand how DNA-dependent processes are regulated.
Recent technological advances made such analyses feasible on a genome-wide scale
at high resolution. In addition, we have recently developed a method to measure
nuclease accessibility of nucleosomes on a global scale. This unit describes
methods to map nucleosome positions, to determine nucleosome density, and to
determine nuclease accessibility of nucleosomes using deep sequencing.