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10.1021/ja505733u http://scihub22266oqcxt.onion/10.1021/ja505733u C4183661!4183661!25188011     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Am+Chem+Soc 2014 ; 136 (39): 13715-26 Nephropedia Template TP {{tp|p=25188011|t=2014. A Short DNA Sequence Confers Strong Bleomycin Binding to Hairpin DNAs |pdf=|usr=}}{{25188011}} gab.com Text A Short DNA Sequence Confers Strong Bleomycin Binding to Hairpin DNAs JO: J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=25188011 #FOAvip Bleomycins A5 and B2 were used to study the structural features in hairpin DNAs conducive to strong BLM?DNA interaction. Two members of a 10-hairpin DNA library previously found to bind most tightly to these BLMs were subsequently noted to share the sequence 5?-ACGC (complementary strand sequence 5?-GCGT). Each underwent double-strand cleavage at five sites within, or near, an eight base pair region of the DNA duplex which had been randomized to create the original library. A new hairpin DNA library was selected based on affinity for immobilized Fe(III)·BLM A5. Two of the 30 newly identified DNAs also contained the sequence 5?-ACGC/5?-GCGT. These DNAs bound to the Fe(II)·BLMs more tightly than any DNA characterized previously. Surface plasmon resonance confirmed tight Fe(III)·BLM B2 binding and gave an excellent fit for a 1:1 binding model, implying the absence of significant secondary binding sites. Fe(II)·BLM A5 was used to assess sites of double-strand DNA cleavage. Both hairpin DNAs underwent double-strand cleavage at five sites within or near the original randomized eight base region. For DNA 12, four of the five double-strand cleavages involved independent single-strand cleavage reactions; DNA 13 underwent double-strand DNA cleavage by independent single-strand cleavages at all five sites. DNA 14, which bound Fe·BLM poorly, was converted to a strong binder (DNA 15) by insertion of the sequence 5?-ACGC/5?-GCGT. These findings reinforce the idea that tighter DNA binding by Fe·BLM leads to increased double-strand cleavage by a novel mechanism and identify a specific DNA motif conducive to strong BLM binding and cleavage. Twit Text FOAVip A Short DNA Sequence Confers Strong Bleomycin Binding to Hairpin DNAs ????J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=25188011 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25188011 #FOAvip English Wikipedia <ref>{{Cite pmid|25188011}}</ref> A Short DNA Sequence Confers Strong Bleomycin Binding to Hairpin DNAs #MMPMID25188011 Tang C; Paul A; Alam M; Roy B; Wilson WD; Hecht SM J Am Chem Soc 2014[Oct]; 136 (39): 13715-26 PMID25188011show ga Bleomycins A5 and B2 were used to study the structural features in hairpin DNAs conducive to strong BLM?DNA interaction. Two members of a 10-hairpin DNA library previously found to bind most tightly to these BLMs were subsequently noted to share the sequence 5?-ACGC (complementary strand sequence 5?-GCGT). Each underwent double-strand cleavage at five sites within, or near, an eight base pair region of the DNA duplex which had been randomized to create the original library. A new hairpin DNA library was selected based on affinity for immobilized Fe(III)·BLM A5. Two of the 30 newly identified DNAs also contained the sequence 5?-ACGC/5?-GCGT. These DNAs bound to the Fe(II)·BLMs more tightly than any DNA characterized previously. Surface plasmon resonance confirmed tight Fe(III)·BLM B2 binding and gave an excellent fit for a 1:1 binding model, implying the absence of significant secondary binding sites. Fe(II)·BLM A5 was used to assess sites of double-strand DNA cleavage. Both hairpin DNAs underwent double-strand cleavage at five sites within or near the original randomized eight base region. For DNA 12, four of the five double-strand cleavages involved independent single-strand cleavage reactions; DNA 13 underwent double-strand DNA cleavage by independent single-strand cleavages at all five sites. DNA 14, which bound Fe·BLM poorly, was converted to a strong binder (DNA 15) by insertion of the sequence 5?-ACGC/5?-GCGT. These findings reinforce the idea that tighter DNA binding by Fe·BLM leads to increased double-strand cleavage by a novel mechanism and identify a specific DNA motif conducive to strong BLM binding and cleavage. äA Short DNA Sequence Confers Strong Bleomycin Binding to Hairpin DNAs (epmc).pdf DeepDyve Pubget Overpricing