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10.4161/rna.29624 http://scihub22266oqcxt.onion/10.4161/rna.29624 C4179957!4179957!25137067     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RNA+Biol 2014 ; 11 (7): 829-35 Nephropedia Template TP {{tp|p=25137067|t=2014. Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9 |pdf=|usr=}}{{25137067}} gab.com Text Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9 JO: RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=25137067 #FOAvip Recent genome-wide studies have revealed that the majority of the mouse genome is transcribed as non-coding RNAs (ncRNAs) and growing evidence supports the importance of ncRNAs in regulating gene expression and epigenetic processes. However, the low efficiency of conventional gene targeting strategies has hindered the functional study of ncRNAs in vivo, particularly in generating large fragment deletions of long non-coding RNAs (lncRNAs) with multiple expression variants. The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system has recently been applied as an efficient tool for engineering site-specific mutations of protein-coding genes in the genome. In this study, we explored the potential of using the CRISPR/Cas9 system to generate large genomic deletions of lncRNAs in mice. We developed an efficient one-step strategy to target the maternally expressed lncRNA, Rian, on chromosome 12 in mice. We showed that paired sgRNAs can precisely generate large deletions up to 23kb and the deletion efficiency can be further improved up to 33% by combining multiple sgRNAs. The deletion successfully abolished the expression of Rian from the maternally inherited allele, validating the biological relevance of the mutations in studying an imprinted locus. Mutation of Rian has differential effects on expression of nearby genes in different somatic tissues. Taken together, we have established a robust one-step method to engineer large deletions to knockout lncRNA genes with the CRISPR/Cas9 system. Our work will facilitate future functional studies of other lncRNAs in vivo. Twit Text FOAVip Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9 ????RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=25137067 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25137067 #FOAvip English Wikipedia <ref>{{Cite pmid|25137067}}</ref> Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9 #MMPMID25137067 Han J; Zhang J; Chen L; Shen B; Zhou J; Hu B; Du Y; Tate PH; Huang X; Zhang W RNA Biol 2014[Jul]; 11 (7): 829-35 PMID25137067show ga Recent genome-wide studies have revealed that the majority of the mouse genome is transcribed as non-coding RNAs (ncRNAs) and growing evidence supports the importance of ncRNAs in regulating gene expression and epigenetic processes. However, the low efficiency of conventional gene targeting strategies has hindered the functional study of ncRNAs in vivo, particularly in generating large fragment deletions of long non-coding RNAs (lncRNAs) with multiple expression variants. The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system has recently been applied as an efficient tool for engineering site-specific mutations of protein-coding genes in the genome. In this study, we explored the potential of using the CRISPR/Cas9 system to generate large genomic deletions of lncRNAs in mice. We developed an efficient one-step strategy to target the maternally expressed lncRNA, Rian, on chromosome 12 in mice. We showed that paired sgRNAs can precisely generate large deletions up to 23kb and the deletion efficiency can be further improved up to 33% by combining multiple sgRNAs. The deletion successfully abolished the expression of Rian from the maternally inherited allele, validating the biological relevance of the mutations in studying an imprinted locus. Mutation of Rian has differential effects on expression of nearby genes in different somatic tissues. Taken together, we have established a robust one-step method to engineer large deletions to knockout lncRNA genes with the CRISPR/Cas9 system. Our work will facilitate future functional studies of other lncRNAs in vivo. äEfficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9 (epmc).pdf DeepDyve Pubget Overpricing