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2014 ; 15
(5
): 1263-74
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Vancomycin-eluting niosomes: a new approach to the inhibition of staphylococcal
biofilm on abiotic surfaces
#MMPMID24895077
AAPS PharmSciTech
2014[Oct]; 15
(5
): 1263-74
PMID24895077
show ga
A new vancomycin (VCM)-eluting mixed bilayer niosome formulation was evaluated
for the control of staphylococcal colonization and biofilm formation on abiotic
surfaces, a niosome application not explored to date. Cosurfactant niosomes were
prepared using a Span 60/Tween 40/cholesterol blend (1: 1: 2). Tween 40, a
polyethoxylated amphiphile, was included to enhance VCM entrapment and confer
niosomal surface properties precluding bacterial adhesion. VCM-eluting niosomes
showed good quality attributes including relatively high entrapment efficiency
(?50%), association of Tween 40 with vesicles in a constant proportion (?87%),
biphasic release profile suitable for inhibiting early bacterial colonization,
and long-term stability at 4°C for a 12-month study period. Niosomes
significantly enhanced VCM activity against planktonic bacteria of nine
staphylococcal strains. Using microtiter plates as abiotic surface, VCM-eluting
niosomes proved superior to VCM in inhibiting biofilm formation, eradicating
surface-borne biofilms, inhibiting biofilm growth, and interfering with biofilm
induction by VCM subminimal inhibitory concentrations. Data suggest dual
functionality of cosurfactant VCM-eluting niosomes as passive colonization
inhibiting barrier and active antimicrobial-controlled delivery system, two
functions recognized in infection control of abiotic surfaces and medical
devices.