Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.jneumeth.2014.04.024 http://scihub22266oqcxt.onion/10.1016/j.jneumeth.2014.04.024 C4179448!4179448!24792527     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24792527&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Neurosci+Methods 2014 ; 232 (ä): 58-62 Nephropedia Template TP {{tp|p=24792527|t=2014. An Open-Source Analytical Platform for Analysis of C elegans Swimming Induced Paralysis |pdf=|usr=}}{{24792527}} gab.com Text An Open-Source Analytical Platform for Analysis of C elegans Swimming Induced Paralysis JO: J Neurosci Methods http://www.kidney.de/pget.php?&tw=1&pmid=24792527 #FOAvip Background: The nematode Caenhorhabditis elegans offers great power for the identification and characterization of genes that regulate behavior. In support of this effort, analytical methods are required that provide dimensional analyses of subcomponents of behavior. Previously, we demonstrated that loss of the presynaptic dopamine (DA) transporter, dat-1, evokes DA-dependent Swimming Induced Paralysis (Swip) (Mcdonald et al. 2007), a behavior compatible with forward genetic screens (Hardaway et al. 2012). New Method: Here, we detail the development and implementation of SwimR, a set of tools that provide for an automated, kinetic analysis of C. elegans Swip. SwimR relies on open source programs that can be freely implemented and modified. Results: We show that SwimR can display time-dependent alterations of swimming behavior induced by drug-treatment, illustrating this capacity with the dat-1 blocker and tricyclic antidepressant imipramine (IMI). We demonstrate the capacity of SwimR to extract multiple kinetic parameters that are impractical to obtain in manual assays. Comparison with Existing Methods: Standard measurements of C. elegans swimming utilizes manual assessments of the number of animals exhibiting swimming versus paralysis. Our approach deconstructs the time course and rates of movement in an automated fashion, offering a significant increase in the information that can be obtained from swimming behavior. Conclusions: The SwimR platform is a powerful tool for the deconstruction of worm thrashing behavior in the context of both genetic and pharmacological manipulations that can be used to segregate pathways that underlie nematode swimming mechanics. Twit Text FOAVip An Open-Source Analytical Platform for Analysis of C elegans Swimming Induced Paralysis ????J Neurosci Methods http://www.kidney.de/pget.php?&tw=1&pmid=24792527 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24792527 #FOAvip English Wikipedia <ref>{{Cite pmid|24792527}}</ref> An Open-Source Analytical Platform for Analysis of C elegans Swimming Induced Paralysis #MMPMID24792527 Hardaway JA; Wang J; Fleming PA; Fleming KA; Whitaker SM; Nackenoff A; Snarrenberg CL; Hardie SL; Zhang B; Blakely RD J Neurosci Methods 2014[Jul]; 232 (ä): 58-62 PMID24792527show ga Background: The nematode Caenhorhabditis elegans offers great power for the identification and characterization of genes that regulate behavior. In support of this effort, analytical methods are required that provide dimensional analyses of subcomponents of behavior. Previously, we demonstrated that loss of the presynaptic dopamine (DA) transporter, dat-1, evokes DA-dependent Swimming Induced Paralysis (Swip) (Mcdonald et al. 2007), a behavior compatible with forward genetic screens (Hardaway et al. 2012). New Method: Here, we detail the development and implementation of SwimR, a set of tools that provide for an automated, kinetic analysis of C. elegans Swip. SwimR relies on open source programs that can be freely implemented and modified. Results: We show that SwimR can display time-dependent alterations of swimming behavior induced by drug-treatment, illustrating this capacity with the dat-1 blocker and tricyclic antidepressant imipramine (IMI). We demonstrate the capacity of SwimR to extract multiple kinetic parameters that are impractical to obtain in manual assays. Comparison with Existing Methods: Standard measurements of C. elegans swimming utilizes manual assessments of the number of animals exhibiting swimming versus paralysis. Our approach deconstructs the time course and rates of movement in an automated fashion, offering a significant increase in the information that can be obtained from swimming behavior. Conclusions: The SwimR platform is a powerful tool for the deconstruction of worm thrashing behavior in the context of both genetic and pharmacological manipulations that can be used to segregate pathways that underlie nematode swimming mechanics. äAn Open-Source Analytical Platform for Analysis of C elegans Swimming(epmc).pdf DeepDyve Pubget Overpricing