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10.1111/exd.12389 http://scihub22266oqcxt.onion/10.1111/exd.12389 C4176812!4176812 !25234828     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25234828 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Exp+Dermatol 2014 ; 23 Suppl 1 (0 1 ): 2-6 Nephropedia Template TP {{tp|p=25234828 |t=2014. Ultraviolet irradiation represses TGF-? type II receptor transcription through a 38-bp sequence in the proximal promoter in human skin fibroblasts |pdf=|usr=}}{{25234828 }} gab.com Text Ultraviolet irradiation represses TGF- type II receptor transcription through a 38-bp sequence in the proximal promoter in human skin fibroblasts JO: Exp Dermatol http://www.kidney.de/pget.php?&tw=1&pmid=25234828 #FOAvip Transforming growth factor-? (TGF-?) is a major regulator of collagen gene expression in human skin fibroblasts. Cellular responses to TGF-? are mediated primarily through its cell surface type I (T?RI) and type II (T?RII) receptors. Ultraviolet (UV) irradiation impairs TGF-? signalling largely due to reduced T?RII gene expression, thereby decreasing type I procollagen synthesis, in human skin fibroblasts. UV irradiation does not alter either T?RII mRNA or protein stability, indicating that UV reduction in T?RII expression likely results from transcriptional or translational repression. To understand how UV irradiation regulates T?RII transcription, we used a series of T?RII promoter-luciferase 5'-deletion constructs (covering 2 kb of the T?RII proximal promoter) to determine transcriptional rate in response to UV irradiation. We identified a 137-bp region upstream of the transcriptional start site that exhibited high promoter activity and was repressed 60% by UV irradiation, whereas all other T?RII promoter reporter constructs exhibited either low promoter activities or no regulation by UV irradiation. Mutation of potential transcription factor binding sites within the promoter region revealed that an inverted CCAAT box (-81 bp from transcription start site) is required for promoter activity. Mutation of the CCAAT box completely abolished UV irradiation regulation of the T?RII promoter. Protein-binding assay, as determined by electrophoretic mobility-shift assays (EMSAs) using the inverted CCAAT box as probe (-100/-62), demonstrated significantly enhanced protein binding in response to UV irradiation. Super shift experiments indicated that nuclear factor Y (NFY) is able to binding to this sequence, but NFY binding was not altered in response to UV irradiation, indicating additional protein(s) are capable of binding this sequence in response to UV irradiation. Taken together, these data indicate that UV irradiation reduces T?RII expression, at least partially, through transcriptional repression. This repression is mediated by a 38-bp sequence in T?RII promoter, in human skin fibroblasts. Twit Text FOAVip Ultraviolet irradiation represses TGF- type II receptor transcription through a 38-bp sequence in the proximal promoter in human skin fibroblasts ????Exp Dermatol http://www.kidney.de/pget.php?&tw=1&pmid=25234828 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25234828 #FOAvip English Wikipedia <ref>{{Cite pmid|25234828 }}</ref> Ultraviolet irradiation represses TGF-? type II receptor transcription through a 38-bp sequence in the proximal promoter in human skin fibroblasts #MMPMID25234828 He T ; Quan T ; Fisher GJ Exp Dermatol 2014[Oct]; 23 Suppl 1 (0 1 ): 2-6 PMID25234828 show ga Transforming growth factor-? (TGF-?) is a major regulator of collagen gene expression in human skin fibroblasts. Cellular responses to TGF-? are mediated primarily through its cell surface type I (T?RI) and type II (T?RII) receptors. Ultraviolet (UV) irradiation impairs TGF-? signalling largely due to reduced T?RII gene expression, thereby decreasing type I procollagen synthesis, in human skin fibroblasts. UV irradiation does not alter either T?RII mRNA or protein stability, indicating that UV reduction in T?RII expression likely results from transcriptional or translational repression. To understand how UV irradiation regulates T?RII transcription, we used a series of T?RII promoter-luciferase 5'-deletion constructs (covering 2 kb of the T?RII proximal promoter) to determine transcriptional rate in response to UV irradiation. We identified a 137-bp region upstream of the transcriptional start site that exhibited high promoter activity and was repressed 60% by UV irradiation, whereas all other T?RII promoter reporter constructs exhibited either low promoter activities or no regulation by UV irradiation. Mutation of potential transcription factor binding sites within the promoter region revealed that an inverted CCAAT box (-81 bp from transcription start site) is required for promoter activity. Mutation of the CCAAT box completely abolished UV irradiation regulation of the T?RII promoter. Protein-binding assay, as determined by electrophoretic mobility-shift assays (EMSAs) using the inverted CCAAT box as probe (-100/-62), demonstrated significantly enhanced protein binding in response to UV irradiation. Super shift experiments indicated that nuclear factor Y (NFY) is able to binding to this sequence, but NFY binding was not altered in response to UV irradiation, indicating additional protein(s) are capable of binding this sequence in response to UV irradiation. Taken together, these data indicate that UV irradiation reduces T?RII expression, at least partially, through transcriptional repression. This repression is mediated by a 38-bp sequence in T?RII promoter, in human skin fibroblasts. |Base Sequence [MESH] |Binding Sites/genetics [MESH] |CCAAT-Binding Factor/antagonists & inhibitors/genetics/metabolism [MESH] |Cells, Cultured [MESH] |Down-Regulation/radiation effects [MESH] |Fibroblasts/metabolism/radiation effects [MESH] |Humans [MESH] |Models, Genetic [MESH] |Mutagenesis, Site-Directed [MESH] |Promoter Regions, Genetic/radiation effects [MESH] |Protein Binding/radiation effects [MESH] |Protein Serine-Threonine Kinases/*genetics [MESH] |RNA, Small Interfering/genetics [MESH] |Receptor, Transforming Growth Factor-beta Type II [MESH] |Receptors, Transforming Growth Factor beta/*genetics [MESH] |Skin/*metabolism/*radiation effects [MESH]Ultraviolet irradiation represses TGF-? type II receptor transcription(epmc).pdf DeepDyve Pubget Overpricing