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2014 ; 6
(20
): 8180-8186
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A Simple Microfluidic Electrochemical HPLC Detector for Quantifying Fenton
Reactivity from Welding Fumes
#MMPMID25267929
Pluangklang T
; Wydallis JB
; Cate DM
; Nacapricha D
; Henry CS
Anal Methods
2014[Oct]; 6
(20
): 8180-8186
PMID25267929
show ga
Development and characterization of a simple microfluidic electrochemical flow
cell that can be coupled with HPLC to enable dual absorbance/electrochemical
detection is described. Coupling absorbance and electrochemical detection
increases the information that can be gathered from a single injection, but a
second (typically expensive) detection system is required. Here, an inexpensive,
customizable microfluidic electrochemical detector is coupled in series with a
commercial HPLC/UV system. The microfluidic device is made from
poly(dimethylsiloxane) and contains carbon paste electrodes. To demonstrate the
utility of this dual-detection system, the reaction products of the radical
scavenging agent salicylic acid and hydroxyl radical generated by Fenton
chemistry were analyzed. The dual-detection system was used to quantify
2,5-dihydroxybenzoic acid, 2,3-dihydroxybenzoic acid, and catechol produced by
the addition of H(2)O(2) to filter samples of welding fumes. Measurement recovery
was high, with percent recoveries between 97-102%, 92-103%, and 95-103% for
2,5-dihydroxybenzoic acid, 2,3-dihydroxybenzoic acid, and catechol, respectively,
for control samples. The methods described in this work are simple, reliable, and
can inexpensively couple electrochemical detection to HPLC-UV systems.