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10.1016/j.ymeth.2014.06.014 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2014.06.014 C4175060!4175060!25010559     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods 2014 ; 69 (2): 188-97 Nephropedia Template TP {{tp|p=25010559|t=2014. Engineering Synthetic TALE and CRISPR/Cas9 Transcription Factors for Regulating Gene Expression |pdf=|usr=}}{{25010559}} gab.com Text Engineering Synthetic TALE and CRISPR/Cas9 Transcription Factors for Regulating Gene Expression JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=25010559 #FOAvip Engineered DNA-binding proteins that can be targeted to specific sites in the genome to manipulate gene expression have enabled many advances in biomedical research. This includes generating tools to study fundamental aspects of gene regulation and the development of a new class of gene therapies that alter the expression of endogenous genes. Designed transcription factors have entered clinical trials for the treatment of human diseases and others are in preclinical development. High-throughput and user-friendly platforms for designing synthetic DNA-binding proteins present innovative methods for deciphering cell biology and designing custom synthetic gene circuits. We review two platforms for designing synthetic transcription factors for manipulating gene expression: Transcription Activator-Like Effectors (TALEs) and the RNA-guided Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system. We present an overview of each technology and a guide for designing and assembling custom TALE- and CRISPR/Cas9-based transcription factors. We also discuss characteristics of each platform that are best suited for different applications. Twit Text FOAVip Engineering Synthetic TALE and CRISPR/Cas9 Transcription Factors for Regulating Gene Expression ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=25010559 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25010559 #FOAvip English Wikipedia <ref>{{Cite pmid|25010559}}</ref> Engineering Synthetic TALE and CRISPR/Cas9 Transcription Factors for Regulating Gene Expression #MMPMID25010559 Kabadi AM; Gersbach CA Methods 2014[Sep]; 69 (2): 188-97 PMID25010559show ga Engineered DNA-binding proteins that can be targeted to specific sites in the genome to manipulate gene expression have enabled many advances in biomedical research. This includes generating tools to study fundamental aspects of gene regulation and the development of a new class of gene therapies that alter the expression of endogenous genes. Designed transcription factors have entered clinical trials for the treatment of human diseases and others are in preclinical development. High-throughput and user-friendly platforms for designing synthetic DNA-binding proteins present innovative methods for deciphering cell biology and designing custom synthetic gene circuits. We review two platforms for designing synthetic transcription factors for manipulating gene expression: Transcription Activator-Like Effectors (TALEs) and the RNA-guided Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system. We present an overview of each technology and a guide for designing and assembling custom TALE- and CRISPR/Cas9-based transcription factors. We also discuss characteristics of each platform that are best suited for different applications. äEngineering Synthetic TALE and CRISPR/Cas9 Transcription Factors for R(epmc).pdf DeepDyve Pubget Overpricing