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2014 ; 20
(10
): 1499-506
Nephropedia Template TP
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English Wikipedia
Chloroplast RNA editing going extreme: more than 3400 events of C-to-U editing in
the chloroplast transcriptome of the lycophyte Selaginella uncinata
#MMPMID25142065
Oldenkott B
; Yamaguchi K
; Tsuji-Tsukinoki S
; Knie N
; Knoop V
RNA
2014[Oct]; 20
(10
): 1499-506
PMID25142065
show ga
RNA editing in chloroplasts and mitochondria of land plants differs significantly
in abundance. For example, some 200-500 sites of cytidine-to-uridine RNA editing
exist in flowering plant mitochondria as opposed to only 30-50 such C-to-U
editing events in their chloroplasts. In contrast, we predicted significantly
more chloroplast RNA editing for the protein-coding genes in the available
complete plastome sequences of two species of the spike moss genus Selaginella
(Lycopodiophyta). To evaluate these predictions we investigated the Selaginella
uncinata chloroplast transcriptome. Our exhaustive cDNA studies identified the
extraordinary number of 3415 RNA-editing events, exclusively of the C-to-U type,
in the 74 mRNAs encoding intact reading frames in the S. uncinata chloroplast. We
find the overwhelming majority (61%) of the 428 silent editing events leaving
codon meanings unaltered directly neighboring other editing events, possibly
suggesting a sterically more flexible RNA-editing deaminase activity in
Selaginella. No evidence of RNA editing was found for tRNAs or rRNAs but we
identified a total of 74 editing sites in cDNA sequences of four group II introns
(petBi6g2, petDi8g2, ycf3i124g2, and ycf3i354g2) retained in partially matured
transcripts, which strongly contribute to improved base-pairing in the intron
secondary structures as a likely prerequisite for their splicing.