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The position- and lymphatic lumen-controlled tissue chambers to study live
lymphatic vessels and surrounding tissues ex vivo
#MMPMID25229433
Maejima D
; Nagai T
; Bridenbaugh EA
; Cromer WE
; Gashev AA
Lymphat Res Biol
2014[Sep]; 12
(3
): 150-6
PMID25229433
show ga
BACKGROUND: Until now, there has been no tool available to provide lymphatic
researchers the ability to perform experiments in tissue explants containing
lymphatic vessels under tissue position- and lymphatic lumen-controlled
conditions. METHODS AND RESULTS: In this article we provide technical details and
description of the method of using the newly developed and implemented the
position- and lymphatic lumen-controlled tissue chambers to study live lymphatic
vessels and surrounding tissues ex vivo. In this study, we, for the first time,
performed detailed comparative analysis of the contractile and pumping activity
of rat mesenteric lymphatic vessels (MLVs) situated within tissue explants
mounted in new tissue chambers and isolated, cannulated, and pressurized rat MLVs
maintained in isolated vessel setups. We found no significant differences of the
effects of both transmural pressure- and wall shear stress sensitivities of MLVs
in tissue chambers and isolated MLVs. CONCLUSIONS: We conclude that this new
experimental tool, a position- and lymphatic lumen-controlled tissue chamber,
allows precise investigation of lymphatic function of MLVs interacting with
elements of the tissue microenvironment. This method provides an important new
set of experimental tools to investigate lymphatic function.
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