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10.1016/j.molcel.2014.07.017 http://scihub22266oqcxt.onion/10.1016/j.molcel.2014.07.017 C4169771!4169771!25175028     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell 2014 ; 55 (6): 868-79 Nephropedia Template TP {{tp|p=25175028|t=2014. A MicroRNA Precursor Surveillance System in Quality Control of MicroRNA Synthesis |pdf=|usr=}}{{25175028}} gab.com Text A MicroRNA Precursor Surveillance System in Quality Control of MicroRNA Synthesis JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=25175028 #FOAvip MicroRNAs (miRNAs) are essential for regulation of gene expression. Though numerous miRNAs have been identified by high throughput sequencing, few precursor miRNAs (pre-miRNAs) are experimentally validated. Here we report a strategy for constructing high-throughput sequencing libraries enriched for full-length pre-miRNAs. We find widespread and extensive uridylation of Argonaute bound pre-miRNAs, which is primarily catalyzed by two terminal uridylyltransferases: TUT7 and TUT4. Uridylation by TUT7/4 not only polishes pre-miRNA 3? ends, but also facilitates their degradation by the exosome, preventing clogging of Ago with defective species. We show that the exosome exploits distinct substrate preferences of DIS3 and RRP6, its two catalytic subunits, to distinguish productive from defective pre-miRNAs. Furthermore, we identify a positive feedback loop formed by the exosome and TUT7/4 in triggering uridylation and degradation of Ago-bound pre-miRNAs. Our study reveals a pre-miRNA surveillance system that comprises TUT7, TUT4 and the exosome in quality control of miRNA synthesis. Twit Text FOAVip A MicroRNA Precursor Surveillance System in Quality Control of MicroRNA Synthesis ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=25175028 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25175028 #FOAvip English Wikipedia <ref>{{Cite pmid|25175028}}</ref> A MicroRNA Precursor Surveillance System in Quality Control of MicroRNA Synthesis #MMPMID25175028 Liu X; Zheng Q; Vrettos N; Maragkakis M; Alexiou P; Gregory BD; Mourelatos Z Mol Cell 2014[Sep]; 55 (6): 868-79 PMID25175028show ga MicroRNAs (miRNAs) are essential for regulation of gene expression. Though numerous miRNAs have been identified by high throughput sequencing, few precursor miRNAs (pre-miRNAs) are experimentally validated. Here we report a strategy for constructing high-throughput sequencing libraries enriched for full-length pre-miRNAs. We find widespread and extensive uridylation of Argonaute bound pre-miRNAs, which is primarily catalyzed by two terminal uridylyltransferases: TUT7 and TUT4. Uridylation by TUT7/4 not only polishes pre-miRNA 3? ends, but also facilitates their degradation by the exosome, preventing clogging of Ago with defective species. We show that the exosome exploits distinct substrate preferences of DIS3 and RRP6, its two catalytic subunits, to distinguish productive from defective pre-miRNAs. Furthermore, we identify a positive feedback loop formed by the exosome and TUT7/4 in triggering uridylation and degradation of Ago-bound pre-miRNAs. Our study reveals a pre-miRNA surveillance system that comprises TUT7, TUT4 and the exosome in quality control of miRNA synthesis. äA MicroRNA Precursor Surveillance System in Quality Control of MicroRN(epmc).pdf DeepDyve Pubget Overpricing