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10.4161/epi.29919 http://scihub22266oqcxt.onion/10.4161/epi.29919 C4169012!4169012!25147920     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Epigenetics 2014 ; 9 (9): 1207-11 Nephropedia Template TP {{tp|p=25147920|t=2014. A CRISPR-based approach for proteomic analysis of a single genomic locus |pdf=|usr=}}{{25147920}} gab.com Text A CRISPR-based approach for proteomic analysis of a single genomic locus JO: Epigenetics http://www.kidney.de/pget.php?&tw=1&pmid=25147920 #FOAvip Any given chromosomal activity (e.g., transcription) is governed predominantly by the local epiproteome. However, defining local epiproteomes has been limited by a lack of effective technologies to isolate discrete sections of chromatin and to identify with precision specific proteins and histone posttranslational modifications (PTMs). We report the use of the Cas9 and guide RNA (gRNA) components of the CRISPR system for gRNA-directed purification of a discrete section of chromatin. Quantitative mass spectrometry provides for unambiguous identification of proteins and histone PTMs specifically associated with the enriched chromatin. This CRISPR-based Chromatin Affinity Purification with Mass Spectrometry (CRISPR-ChAP-MS) approach revealed changes in the local epiproteome of a promoter during activation of transcription. CRISPR-ChAP-MS thus has broad applications for discovering molecular components and dynamic regulation of any in vivo activity at a given chromosomal location. Twit Text FOAVip A CRISPR-based approach for proteomic analysis of a single genomic locus ????Epigenetics http://www.kidney.de/pget.php?&tw=1&pmid=25147920 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25147920 #FOAvip English Wikipedia <ref>{{Cite pmid|25147920}}</ref> A CRISPR-based approach for proteomic analysis of a single genomic locus #MMPMID25147920 Waldrip ZJ; Byrum SD; Storey AJ; Gao J; Byrd AK; Mackintosh SG; Wahls WP; Taverna SD; Raney KD; Tackett AJ Epigenetics 2014[Sep]; 9 (9): 1207-11 PMID25147920show ga Any given chromosomal activity (e.g., transcription) is governed predominantly by the local epiproteome. However, defining local epiproteomes has been limited by a lack of effective technologies to isolate discrete sections of chromatin and to identify with precision specific proteins and histone posttranslational modifications (PTMs). We report the use of the Cas9 and guide RNA (gRNA) components of the CRISPR system for gRNA-directed purification of a discrete section of chromatin. Quantitative mass spectrometry provides for unambiguous identification of proteins and histone PTMs specifically associated with the enriched chromatin. This CRISPR-based Chromatin Affinity Purification with Mass Spectrometry (CRISPR-ChAP-MS) approach revealed changes in the local epiproteome of a promoter during activation of transcription. CRISPR-ChAP-MS thus has broad applications for discovering molecular components and dynamic regulation of any in vivo activity at a given chromosomal location. äA CRISPR-based approach for proteomic analysis of a single genomic loc(epmc).pdf DeepDyve Pubget Overpricing