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10.1152/ajpcell.00392.2013 http://scihub22266oqcxt.onion/10.1152/ajpcell.00392.2013 C4166739!4166739 !25031022     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25031022 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Am+J+Physiol+Cell+Physiol 2014 ; 307 (6 ): C532-41 Nephropedia Template TP {{tp|p=25031022 |t=2014. Uroguanylin inhibits H-ATPase activity and surface expression in renal distal tubules by a PKG-dependent pathway |pdf=|usr=}}{{25031022 }} gab.com Text Uroguanylin inhibits H-ATPase activity and surface expression in renal distal tubules by a PKG-dependent pathway JO: Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=25031022 #FOAvip Cumulative evidence suggests that guanylin peptides play an important role on electrolyte homeostasis. We have previously reported that uroguanylin (UGN) inhibits bicarbonate reabsorption in a renal distal tubule. In the present study, we tested the hypothesis that the bicarbonaturic effect of UGN is at least in part attributable to inhibition of H(+)-ATPase-mediated hydrogen secretion in the distal nephron. By in vivo stationary microperfusion experiments, we were able to show that UGN inhibits H(+)-ATPase activity by a PKG-dependent pathway because KT5823 (PKG inhibitor) abolished the UGN effect on distal bicarbonate reabsorption and H89 (PKA inhibitor) was unable to prevent it. The in vivo results were confirmed by the in vitro experiments, where we used fluorescence microscopy to measure intracellular pH (pHi) recovery after an acid pulse with NH4Cl. By this technique, we observed that UGN and 8 bromoguanosine-cGMP (8Br-cGMP) inhibited H(+)-ATPase-dependent pHi recovery and that the UGN inhibitory effect was abolished in the presence of the PKG inhibitor. In addition, by using RT-PCR technique, we verified that Madin-Darby canine kidney (MDCK)-C11 cells express guanylate cyclase-C. Besides, UGN stimulated an increase of both cGMP content and PKG activity but was unable to increase the production of cellular cAMP content and PKA activity. Furthermore, we found that UGN reduced cell surface abundance of H+-ATPase B1 subunit in MDCK-C11 and that this effect was abolished by the PKG inhibitor. Taken together, our data suggest that UGN inhibits H(+)-ATPase activity and surface expression in renal distal cells by a cGMP/PKG-dependent pathway. Twit Text FOAVip Uroguanylin inhibits H-ATPase activity and surface expression in renal distal tubules by a PKG-dependent pathway ????Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=25031022 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25031022 #FOAvip English Wikipedia <ref>{{Cite pmid|25031022 }}</ref> Uroguanylin inhibits H-ATPase activity and surface expression in renal distal tubules by a PKG-dependent pathway #MMPMID25031022 da Silva Lima V ; Crajoinas RO ; Carraro-Lacroix LR ; Godinho AN ; Dias JL ; Dariolli R ; Girardi AC ; Fonteles MC ; Malnic G ; Lessa LM Am J Physiol Cell Physiol 2014[Sep]; 307 (6 ): C532-41 PMID25031022 show ga Cumulative evidence suggests that guanylin peptides play an important role on electrolyte homeostasis. We have previously reported that uroguanylin (UGN) inhibits bicarbonate reabsorption in a renal distal tubule. In the present study, we tested the hypothesis that the bicarbonaturic effect of UGN is at least in part attributable to inhibition of H(+)-ATPase-mediated hydrogen secretion in the distal nephron. By in vivo stationary microperfusion experiments, we were able to show that UGN inhibits H(+)-ATPase activity by a PKG-dependent pathway because KT5823 (PKG inhibitor) abolished the UGN effect on distal bicarbonate reabsorption and H89 (PKA inhibitor) was unable to prevent it. The in vivo results were confirmed by the in vitro experiments, where we used fluorescence microscopy to measure intracellular pH (pHi) recovery after an acid pulse with NH4Cl. By this technique, we observed that UGN and 8 bromoguanosine-cGMP (8Br-cGMP) inhibited H(+)-ATPase-dependent pHi recovery and that the UGN inhibitory effect was abolished in the presence of the PKG inhibitor. In addition, by using RT-PCR technique, we verified that Madin-Darby canine kidney (MDCK)-C11 cells express guanylate cyclase-C. Besides, UGN stimulated an increase of both cGMP content and PKG activity but was unable to increase the production of cellular cAMP content and PKA activity. Furthermore, we found that UGN reduced cell surface abundance of H+-ATPase B1 subunit in MDCK-C11 and that this effect was abolished by the PKG inhibitor. Taken together, our data suggest that UGN inhibits H(+)-ATPase activity and surface expression in renal distal cells by a cGMP/PKG-dependent pathway. |Animals [MESH] |Bicarbonates/metabolism [MESH] |Cell Membrane/*drug effects/enzymology [MESH] |Cyclic GMP-Dependent Protein Kinases/antagonists & inhibitors/*metabolism [MESH] |Cyclic GMP/metabolism [MESH] |Dogs [MESH] |Hydrogen-Ion Concentration [MESH] |Kidney Tubules, Distal/*drug effects/enzymology [MESH] |Madin Darby Canine Kidney Cells [MESH] |Male [MESH] |Natriuretic Peptides/*pharmacology [MESH] |Perfusion [MESH] |Protein Kinase Inhibitors/pharmacology [MESH] |Protein Transport [MESH] |Proton-Translocating ATPases/*metabolism [MESH] |Rats [MESH] |Rats, Wistar [MESH] |Receptors, Guanylate Cyclase-Coupled/drug effects/genetics/metabolism [MESH] |Signal Transduction/drug effects [MESH]Uroguanylin inhibits H-ATPase activity and surface expression in renal(epmc).pdf DeepDyve Pubget Overpricing