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10.1117/1.JBO.19.9.096011

http://scihub22266oqcxt.onion/10.1117/1.JBO.19.9.096011
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C4164706!4164706!25223708
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suck abstract from ncbi


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pmid25223708      J+Biomed+Opt 2014 ; 19 (9): ä
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  • Microvascular quantification based on contour-scanning photoacoustic microscopy #MMPMID25223708
  • Yeh C; Soetikno B; Hu S; Maslov KI; Wang LV
  • J Biomed Opt 2014[Sep]; 19 (9): ä PMID25223708show ga
  • Accurate quantification of microvasculature remains of interest in fundamental pathophysiological studies and clinical trials. Current photoacoustic microscopy can noninvasively quantify properties of the microvasculature, including vessel density and diameter, with a high spatial resolution. However, the depth range of focus (i.e., focal zone) of optical-resolution photoacoustic microscopy (OR-PAM) is often insufficient to encompass the depth variations of features of interest?such as blood vessels?due to uneven tissue surfaces. Thus, time-consuming image acquisitions at multiple different focal planes are required to maintain the region of interest in the focal zone. We have developed continuous three-dimensional motorized contour-scanning OR-PAM, which enables real-time adjustment of the focal plane to track the vessels? profile. We have experimentally demonstrated that contour scanning improves the signal-to-noise ratio of conventional OR-PAM by as much as 41% and shortens the image acquisition time by 3.2 times. Moreover, contour-scanning OR-PAM more accurately quantifies vessel density and diameter, and has been applied to studying tumors with uneven surfaces.
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