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2014 ; 19
(9
): 096007
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Texture analysis applied to second harmonic generation image data for ovarian
cancer classification
#MMPMID26296156
Wen BL
; Brewer MA
; Nadiarnykh O
; Hocker J
; Singh V
; Mackie TR
; Campagnola PJ
J Biomed Opt
2014[Sep]; 19
(9
): 096007
PMID26296156
show ga
Remodeling of the extracellular matrix has been implicated in ovarian cancer. To
quantitate the remodeling, we implement a form of texture analysis to delineate
the collagen fibrillar morphology observed in second harmonic generation
microscopy images of human normal and high grade malignant ovarian tissues. In
the learning stage, a dictionary of ?textons??frequently occurring texture
features that are identified by measuring the image response to a filter bank of
various shapes, sizes, and orientations?is created. By calculating a
representative model based on the texton distribution for each tissue type using
a training set of respective second harmonic generation images, we then perform
classification between images of normal and high grade malignant ovarian tissues.
By optimizing the number of textons and nearest neighbors, we achieved
classification accuracy up to 97% based on the area under receiver operating
characteristic curves (true positives versus false positives). The local analysis
algorithm is a more general method to probe rapidly changing fibrillar
morphologies than global analyses such as FFT. It is also more versatile than
other texture approaches as the filter bank can be highly tailored to specific
applications (e.g., different disease states) by creating customized libraries
based on common image features.