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10.1039/c4mt00121d

http://scihub22266oqcxt.onion/10.1039/c4mt00121d
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C4160025!4160025!24992831
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suck abstract from ncbi


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pmid24992831      Metallomics 2014 ; 6 (9): 1648-55
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  • 3D Imaging of Transition Metals in the Zebrafish Embryo by X-ray Fluorescence Microtomography #MMPMID24992831
  • Bourassa D; Gleber SC; Vogt S; Yi H; Will F; Richter H; Shin CH; Fahrni CJ
  • Metallomics 2014[Sep]; 6 (9): 1648-55 PMID24992831show ga
  • Synchrotron X-ray fluorescence (SXRF) microtomography has emerged as a powerful technique for the 3D visualization of the elemental distribution in biological samples. The mechanical stability, both of the instrument and the specimen, is paramount when acquiring tomographic projection series. By combining the progressive lowering of temperature method (PLT) with femtosecond laser sectioning, we were able to embed, excise, and preserve a zebrafish embryo at 24 hours post fertilization in an X-ray compatible, transparent resin for tomographic elemental imaging. Based on a data set comprised of 60 projections, acquired with a step size of 2 ?m during 100 hours of beam time, we reconstructed the 3D distribution of zinc, iron, and copper using the iterative maximum likelihood expectation maximization (MLEM) reconstruction algorithm. The volumetric elemental maps, which entail over 124 million individual voxels for each transition metal, revealed distinct elemental distributions that could be correlated with characteristic anatomical features at this stage of embryonic development.
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