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10.1016/j.bpj.2014.07.042 http://scihub22266oqcxt.onion/10.1016/j.bpj.2014.07.042 C4156671!4156671!25185547     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biophys+J 2014 ; 107 (5): 1117-28 Nephropedia Template TP {{tp|p=25185547|t=2014. Probing ?-310 Transitions in a Voltage-Sensing S4 Helix |pdf=|usr=}}{{25185547}} gab.com Text Probing -310 Transitions in a Voltage-Sensing S4 Helix JO: Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=25185547 #FOAvip The S4 helix of voltage sensor domains (VSDs) transfers its gating charges across the membrane electrical field in response to changes of the membrane potential. Recent studies suggest that this process may occur via the helical conversion of the entire S4 between ? and 310 conformations. Here, using LRET and FRET, we tested this hypothesis by measuring dynamic changes in the transmembrane length of S4 from engineered VSDs expressed in Xenopus oocytes. Our results suggest that the native S4 from the Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) does not exhibit extended and long-lived 310 conformations and remains mostly ?-helical. Although the S4 of NavAb displays a fully extended 310 conformation in x-ray structures, its transplantation in the Ci-VSP VSD scaffold yielded similar results as the native Ci-VSP S4. Taken together, our study does not support the presence of long-lived extended ?-to-310 helical conversions of the S4 in Ci-VSP associated with voltage activation. Twit Text FOAVip Probing -310 Transitions in a Voltage-Sensing S4 Helix ????Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=25185547 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25185547 #FOAvip English Wikipedia <ref>{{Cite pmid|25185547}}</ref> Probing ?-310 Transitions in a Voltage-Sensing S4 Helix #MMPMID25185547 Kubota T; Lacroix J; Bezanilla F; Correa A Biophys J 2014[Sep]; 107 (5): 1117-28 PMID25185547show ga The S4 helix of voltage sensor domains (VSDs) transfers its gating charges across the membrane electrical field in response to changes of the membrane potential. Recent studies suggest that this process may occur via the helical conversion of the entire S4 between ? and 310 conformations. Here, using LRET and FRET, we tested this hypothesis by measuring dynamic changes in the transmembrane length of S4 from engineered VSDs expressed in Xenopus oocytes. Our results suggest that the native S4 from the Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) does not exhibit extended and long-lived 310 conformations and remains mostly ?-helical. Although the S4 of NavAb displays a fully extended 310 conformation in x-ray structures, its transplantation in the Ci-VSP VSD scaffold yielded similar results as the native Ci-VSP S4. Taken together, our study does not support the presence of long-lived extended ?-to-310 helical conversions of the S4 in Ci-VSP associated with voltage activation. äProbing ?-310 Transitions in a Voltage-Sensing S4 Helix (epmc).pdf DeepDyve Pubget Overpricing