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10.1158/2326-6066.CIR-14-0038

http://scihub22266oqcxt.onion/10.1158/2326-6066.CIR-14-0038
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C4156517!4156517!24852944
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suck abstract from ncbi


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pmid24852944      Cancer+Immunol+Res 2014 ; 2 (9): 839-45
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  • Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy #MMPMID24852944
  • Litterman AJ; Zellmer DM; LaRue RS; Jameson SC; Largaespada DA
  • Cancer Immunol Res 2014[Sep]; 2 (9): 839-45 PMID24852944show ga
  • Cytotoxic T cells typically are expanded ex vivo in culture with IL-2 for adoptive immunotherapy. This culture period leads to a differentiated phenotype and acquisition of effector function, as well as a loss of in vivo proliferative capability and antitumor efficacy. Here, we report antigen-specific and polyclonal expansion of cytotoxic T cells in a cocktail of cytokines and small molecules that leads to a memory-like phenotype in mouse and human cells even during extended culture, leading to enhanced in vivo expansion and tumor control in mice.
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