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10.4161/rna.28800 http://scihub22266oqcxt.onion/10.4161/rna.28800 C4156506!4156506!24823909     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RNA+Biol 2014 ; 11 (6): 755-65 Nephropedia Template TP {{tp|p=24823909|t=2014. The RNA-binding protein hnRNPA2 regulates ?-catenin protein expression and is overexpressed in prostate cancer |pdf=|usr=}}{{24823909}} gab.com Text The RNA-binding protein hnRNPA2 regulates -catenin protein expression and is overexpressed in prostate cancer JO: RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=24823909 #FOAvip Introduction The RNA-binding protein hnRNPA2 (HNRNPA2B1) is upregulated in cancer, where it controls alternative pre-mRNA splicing of cancer-relevant genes. Cytoplasmic hnRNPA2 is reported in aggressive cancers, but is functionally uncharacterized. We explored the role of hnRNPA2 in prostate cancer (PCa). Methods: hnRNPA2 function/localization/expression in PCa was determined using biochemical approaches (colony forming/proliferation/luciferase reporter assays/flow cytometry/immunohistocytochemistry). Binding of hnRNPA2 within cancer-relevant 3?-UTR mRNAs was identified by bioinformatics. Results: RNAi-mediated knockdown of hnRNPA2 reduced colony forming and proliferation, while hnRNPA2 overexpression increased proliferation of PCa cells. Nuclear hnRNPA2 is overexpressed in high-grade clinical PCa, and is also observed in the cytoplasm in some cases. Ectopic expression of a predominantly cytoplasmic variant hnRNPA2-?RGG also increased PCa cell proliferation, suggesting that cytoplasmic hnRNPA2 may also be functionally relevant in PCa. Consistent with its known cytoplasmic roles, hnRNPA2 was associated with 3?-UTR mRNAs of several cancer-relevant mRNAs including ?-catenin (CTNNB1). Both wild-type hnRNPA2 and hnRNPA2-?RGG act on CTNNB1 3?-UTR mRNA, increasing endogenous CTNNB1 mRNA expression and ?-catenin protein expression and nuclear localization. Conclusion: Nuclear and cytoplasmic hnRNPA2 are present in PCa and appear to be functionally important. Cytoplasmic hnRNPA2 may affect the cancer cell phenotype through 3?-UTR mRNA-mediated regulation of ?-catenin expression and other cancer-relevant genes. Twit Text FOAVip The RNA-binding protein hnRNPA2 regulates -catenin protein expression and is overexpressed in prostate cancer ????RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=24823909 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24823909 #FOAvip English Wikipedia <ref>{{Cite pmid|24823909}}</ref> The RNA-binding protein hnRNPA2 regulates ?-catenin protein expression and is overexpressed in prostate cancer #MMPMID24823909 Stockley J; Villasevil MEM; Nixon C; Ahmad I; Leung HY; Rajan P RNA Biol 2014[Jun]; 11 (6): 755-65 PMID24823909show ga Introduction The RNA-binding protein hnRNPA2 (HNRNPA2B1) is upregulated in cancer, where it controls alternative pre-mRNA splicing of cancer-relevant genes. Cytoplasmic hnRNPA2 is reported in aggressive cancers, but is functionally uncharacterized. We explored the role of hnRNPA2 in prostate cancer (PCa). Methods: hnRNPA2 function/localization/expression in PCa was determined using biochemical approaches (colony forming/proliferation/luciferase reporter assays/flow cytometry/immunohistocytochemistry). Binding of hnRNPA2 within cancer-relevant 3?-UTR mRNAs was identified by bioinformatics. Results: RNAi-mediated knockdown of hnRNPA2 reduced colony forming and proliferation, while hnRNPA2 overexpression increased proliferation of PCa cells. Nuclear hnRNPA2 is overexpressed in high-grade clinical PCa, and is also observed in the cytoplasm in some cases. Ectopic expression of a predominantly cytoplasmic variant hnRNPA2-?RGG also increased PCa cell proliferation, suggesting that cytoplasmic hnRNPA2 may also be functionally relevant in PCa. Consistent with its known cytoplasmic roles, hnRNPA2 was associated with 3?-UTR mRNAs of several cancer-relevant mRNAs including ?-catenin (CTNNB1). Both wild-type hnRNPA2 and hnRNPA2-?RGG act on CTNNB1 3?-UTR mRNA, increasing endogenous CTNNB1 mRNA expression and ?-catenin protein expression and nuclear localization. Conclusion: Nuclear and cytoplasmic hnRNPA2 are present in PCa and appear to be functionally important. Cytoplasmic hnRNPA2 may affect the cancer cell phenotype through 3?-UTR mRNA-mediated regulation of ?-catenin expression and other cancer-relevant genes. äThe RNA-binding protein hnRNPA2 regulates ?-catenin protein expression(epmc).pdf DeepDyve Pubget Overpricing