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10.1016/j.surg.2014.04.014 http://scihub22266oqcxt.onion/10.1016/j.surg.2014.04.014 C4150742!4150742 !24882759     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24882759 &cmd=llinks): Failed to open stream: HTTP request failed! 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Exosomes mediate intercellular transfer of pro-fibrogenic connective tissue growth factor (CCN2) between hepatic stellate cells, the principal fibrotic cells in the liver |pdf=|usr=}}{{24882759 }} gab.com Text Exosomes mediate intercellular transfer of pro-fibrogenic connective tissue growth factor (CCN2) between hepatic stellate cells, the principal fibrotic cells in the liver JO: Surgery http://www.kidney.de/pget.php?&tw=1&pmid=24882759 #FOAvip BACKGROUND: Fibrogenic pathways in the liver are principally regulated by hepatic stellate cells (HSC), which produce and respond to fibrotic mediators such as connective tissue growth factor (CCN2). The aim of this study was to determine whether CCN2 is shuttled between HSC in membranous nanovesicles, or "exosomes." METHODS: Exosomes were incubated with HSC after isolation from conditioned medium of control or CCN2-green fluorescent protein (GFP)-transfected primary mouse HSC or human LX-2 HSC. Some exosomes were stained fluorescently with PKH26. HSC co-culture experiments were performed in the presence of GW4869 exosome inhibitor. CCN2 or CCN2-GFP were evaluated by quantitative real-time polymerase chain reaction or Western blot. RESULTS: HSC-derived exosomes contained CCN2 or CCN2 mRNA, each of which increased in concentration during HSC activation or after transfection of HSC with CCN2-GFP. Exosomes, stained with either PKH26 or purified from CCN2-GFP-transfected cells, were taken up by activated or quiescent HSC resulting in CCN2-GFP delivery, as shown by their direct addition to recipient cells or by the GW4869-dependency of donor HSC. CONCLUSION: CCN2 is packaged into secreted, nano-sized exosomes that mediate its intercellular transfer between HSC. Exosomal CCN2 may amplify or fine tune fibrogenic signaling and, in conjunction with other exosome constituents, may have utility as a noninvasive biomarker to assess hepatic fibrosis. Twit Text FOAVip Exosomes mediate intercellular transfer of pro-fibrogenic connective tissue growth factor (CCN2) between hepatic stellate cells, the principal fibrotic cells in the liver ????Surgery http://www.kidney.de/pget.php?&tw=1&pmid=24882759 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24882759 #FOAvip English Wikipedia <ref>{{Cite pmid|24882759 }}</ref> Exosomes mediate intercellular transfer of pro-fibrogenic connective tissue growth factor (CCN2) between hepatic stellate cells, the principal fibrotic cells in the liver #MMPMID24882759 Charrier A ; Chen R ; Chen L ; Kemper S ; Hattori T ; Takigawa M ; Brigstock DR Surgery 2014[Sep]; 156 (3 ): 548-55 PMID24882759 show ga BACKGROUND: Fibrogenic pathways in the liver are principally regulated by hepatic stellate cells (HSC), which produce and respond to fibrotic mediators such as connective tissue growth factor (CCN2). The aim of this study was to determine whether CCN2 is shuttled between HSC in membranous nanovesicles, or "exosomes." METHODS: Exosomes were incubated with HSC after isolation from conditioned medium of control or CCN2-green fluorescent protein (GFP)-transfected primary mouse HSC or human LX-2 HSC. Some exosomes were stained fluorescently with PKH26. HSC co-culture experiments were performed in the presence of GW4869 exosome inhibitor. CCN2 or CCN2-GFP were evaluated by quantitative real-time polymerase chain reaction or Western blot. RESULTS: HSC-derived exosomes contained CCN2 or CCN2 mRNA, each of which increased in concentration during HSC activation or after transfection of HSC with CCN2-GFP. Exosomes, stained with either PKH26 or purified from CCN2-GFP-transfected cells, were taken up by activated or quiescent HSC resulting in CCN2-GFP delivery, as shown by their direct addition to recipient cells or by the GW4869-dependency of donor HSC. CONCLUSION: CCN2 is packaged into secreted, nano-sized exosomes that mediate its intercellular transfer between HSC. Exosomal CCN2 may amplify or fine tune fibrogenic signaling and, in conjunction with other exosome constituents, may have utility as a noninvasive biomarker to assess hepatic fibrosis. |Aniline Compounds/pharmacology [MESH] |Animals [MESH] |Benzylidene Compounds/pharmacology [MESH] |Biological Transport, Active/drug effects [MESH] |Cell Line [MESH] |Cells, Cultured [MESH] |Coculture Techniques [MESH] |Connective Tissue Growth Factor/genetics/*metabolism [MESH] |Exosomes/drug effects/*metabolism [MESH] |Hepatic Stellate Cells/drug effects/*metabolism [MESH] |Humans [MESH] |Mice [MESH] |RNA, Messenger/genetics/metabolism [MESH] |Recombinant Fusion Proteins/genetics/metabolism [MESH] |Signal Transduction [MESH]Exosomes mediate intercellular transfer of pro-fibrogenic connective t(epmc).pdf DeepDyve Pubget Overpricing