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10.1242/jcs.149849 http://scihub22266oqcxt.onion/10.1242/jcs.149849 C4150063!4150063!25002405     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Sci 2014 ; 127 (17): 3782-93 Nephropedia Template TP {{tp|p=25002405|t=2014. Palmitoylation of plakophilin is required for desmosome assembly |pdf=|usr=}}{{25002405}} gab.com Text Palmitoylation of plakophilin is required for desmosome assembly JO: J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=25002405 #FOAvip Desmosomes are prominent adhesive junctions found in various epithelial tissues. The cytoplasmic domains of desmosomal cadherins interact with a host of desmosomal plaque proteins, including plakophilins, plakoglobin and desmoplakin, which, in turn, recruit the intermediate filament cytoskeleton to sites of cell?cell contact. Although the individual components of the desmosome are known, mechanisms regulating the assembly of this junction are poorly understood. Protein palmitoylation is a posttranslational lipid modification that plays an important role in protein trafficking and function. Here, we demonstrate that multiple desmosomal components are palmitoylated in vivo. Pharmacologic inhibition of palmitoylation disrupts desmosome assembly at cell?cell borders. We mapped the site of plakophilin palmitoylation to a conserved cysteine residue present in the armadillo repeat domain. Mutation of this single cysteine residue prevents palmitoylation, disrupts plakophilin incorporation into the desmosomal plaque and prevents plakophilin-dependent desmosome assembly. Finally, plakophilin mutants unable to become palmitoylated act in a dominant-negative manner to disrupt proper localization of endogenous desmosome components and decrease desmosomal adhesion. Taken together, these data demonstrate that palmitoylation of desmosomal components is important for desmosome assembly and adhesion. Twit Text FOAVip Palmitoylation of plakophilin is required for desmosome assembly ????J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=25002405 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25002405 #FOAvip English Wikipedia <ref>{{Cite pmid|25002405}}</ref> Palmitoylation of plakophilin is required for desmosome assembly #MMPMID25002405 Roberts BJ; Johnson KE; McGuinn KP; Saowapa J; Svoboda RA; Mahoney MG; Johnson KR; Wahl JK J Cell Sci 2014[Sep]; 127 (17): 3782-93 PMID25002405show ga Desmosomes are prominent adhesive junctions found in various epithelial tissues. The cytoplasmic domains of desmosomal cadherins interact with a host of desmosomal plaque proteins, including plakophilins, plakoglobin and desmoplakin, which, in turn, recruit the intermediate filament cytoskeleton to sites of cell?cell contact. Although the individual components of the desmosome are known, mechanisms regulating the assembly of this junction are poorly understood. Protein palmitoylation is a posttranslational lipid modification that plays an important role in protein trafficking and function. Here, we demonstrate that multiple desmosomal components are palmitoylated in vivo. Pharmacologic inhibition of palmitoylation disrupts desmosome assembly at cell?cell borders. We mapped the site of plakophilin palmitoylation to a conserved cysteine residue present in the armadillo repeat domain. Mutation of this single cysteine residue prevents palmitoylation, disrupts plakophilin incorporation into the desmosomal plaque and prevents plakophilin-dependent desmosome assembly. Finally, plakophilin mutants unable to become palmitoylated act in a dominant-negative manner to disrupt proper localization of endogenous desmosome components and decrease desmosomal adhesion. Taken together, these data demonstrate that palmitoylation of desmosomal components is important for desmosome assembly and adhesion. äPalmitoylation of plakophilin is required for desmosome assembly (epmc).pdf DeepDyve Pubget Overpricing