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10.1016/j.jim.2014.04.011 http://scihub22266oqcxt.onion/10.1016/j.jim.2014.04.011 C4138252!4138252 !24801479     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24801479 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Immunol+Methods 2014 ; 409 (ä): 62-71 Nephropedia Template TP {{tp|p=24801479 |t=2014. Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays |pdf=|usr=}}{{24801479 }} gab.com Text Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays JO: J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=24801479 #FOAvip Luminex bead array assays are widely used for rapid biomarker quantification due to the ability to measure up to 100 unique analytes in a single well of a 96-well plate. There has been, however, no comprehensive analysis of variables impacting assay performance, nor development of a standardized proficiency testing program for laboratories performing these assays. To meet this need, the NIH/NIAID and the Cancer Immunotherapy Consortium of the Cancer Research Institute collaborated to develop and implement a Luminex assay proficiency testing program as part of the NIH/NIAID-sponsored External Quality Assurance Program Oversight Laboratory (EQAPOL) at Duke University. The program currently monitors 25 domestic and international sites with two external proficiency panels per year. Each panel includes a de-identified commercial Luminex assay kit with standards to quantify human IFN?, TNF?, IL-6, IL-10 and IL-2, and a series of recombinant cytokine-spiked human serum samples. All aspects of panel development, testing and shipping are performed under GCLP by EQAPOL support teams. Following development testing, a comprehensive site proficiency scoring system comprised of timeliness, protocol adherence, accuracy and precision was implemented. The overall mean proficiency score across three rounds of testing has remained stable (EP3: 76%, EP4: 75%, EP5: 77%); however, a more detailed analysis of site reported results indicates a significant improvement of intra- (within) and inter- (between) site variation, suggesting that training and remediation for poor performing sites may be having a positive impact on proficiency. Through continued proficiency testing, identification of variables affecting Luminex assay outcomes will strengthen efforts to bring standardization to the field. Twit Text FOAVip Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays ????J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=24801479 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24801479 #FOAvip English Wikipedia <ref>{{Cite pmid|24801479 }}</ref> Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays #MMPMID24801479 Lynch HE ; Sanchez AM ; D'Souza MP ; Rountree W ; Denny TN ; Kalos M ; Sempowski GD J Immunol Methods 2014[Jul]; 409 (ä): 62-71 PMID24801479 show ga Luminex bead array assays are widely used for rapid biomarker quantification due to the ability to measure up to 100 unique analytes in a single well of a 96-well plate. There has been, however, no comprehensive analysis of variables impacting assay performance, nor development of a standardized proficiency testing program for laboratories performing these assays. To meet this need, the NIH/NIAID and the Cancer Immunotherapy Consortium of the Cancer Research Institute collaborated to develop and implement a Luminex assay proficiency testing program as part of the NIH/NIAID-sponsored External Quality Assurance Program Oversight Laboratory (EQAPOL) at Duke University. The program currently monitors 25 domestic and international sites with two external proficiency panels per year. Each panel includes a de-identified commercial Luminex assay kit with standards to quantify human IFN?, TNF?, IL-6, IL-10 and IL-2, and a series of recombinant cytokine-spiked human serum samples. All aspects of panel development, testing and shipping are performed under GCLP by EQAPOL support teams. Following development testing, a comprehensive site proficiency scoring system comprised of timeliness, protocol adherence, accuracy and precision was implemented. The overall mean proficiency score across three rounds of testing has remained stable (EP3: 76%, EP4: 75%, EP5: 77%); however, a more detailed analysis of site reported results indicates a significant improvement of intra- (within) and inter- (between) site variation, suggesting that training and remediation for poor performing sites may be having a positive impact on proficiency. Through continued proficiency testing, identification of variables affecting Luminex assay outcomes will strengthen efforts to bring standardization to the field. |Biomarkers/blood [MESH] |Cooperative Behavior [MESH] |Cytokines/*blood [MESH] |Guideline Adherence/standards [MESH] |High-Throughput Screening Assays/*standards [MESH] |Humans [MESH] |International Cooperation [MESH] |Laboratories/*standards [MESH] |Laboratory Proficiency Testing/*standards [MESH] |Monitoring, Immunologic/*standards [MESH] |Multicenter Studies as Topic/*standards [MESH] |Observer Variation [MESH] |Practice Guidelines as Topic/standards [MESH] |Predictive Value of Tests [MESH] |Program Development [MESH] |Program Evaluation [MESH] |Quality Control [MESH] |Quality Improvement/standards [MESH] |Quality Indicators, Health Care/standards [MESH] |Reference Standards [MESH] |Reproducibility of Results [MESH] |Specimen Handling/standards [MESH] |Time Factors [MESH]Development and implementation of a proficiency testing program for Lu(epmc).pdf DeepDyve Pubget Overpricing