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10.1097/MIB.0000000000000144 http://scihub22266oqcxt.onion/10.1097/MIB.0000000000000144 C4134710!4134710 !25078150     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25078150 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Inflamm+Bowel+Dis 2014 ; 20 (9 ): 1530-47 Nephropedia Template TP {{tp|p=25078150 |t=2014. Implications of the colonic deposition of free hemoglobin-? chain: a previously unknown tissue by-product in inflammatory bowel disease |pdf=|usr=}}{{25078150 }} gab.com Text Implications of the colonic deposition of free hemoglobin- chain: a previously unknown tissue by-product in inflammatory bowel disease JO: Inflamm Bowel Dis http://www.kidney.de/pget.php?&tw=1&pmid=25078150 #FOAvip BACKGROUND: We analyzed inflamed mucosal/submucosal layers of ulcerative colitis (UC = 63) and Crohn's colitis (CC = 50), and unexpectedly, we unveiled a pool of free hemoglobin alpha (Hb-?) chain. Patients with colitides have increased reactive oxidative stress (ROS), DNA oxidation products, free iron in mucosa, in preneoplastic, and in colitis-cancers and increased risks of developing colorectal cancer. All inflammatory bowel disease-related colorectal cancer lesions are found in segments with colitis. Linking this information, we investigated whether free Hb-? is key transformational stepping that increases colitis-related colorectal cancer vulnerability. METHODS: UC/CC samples were profiled using matrix-assisted laser desorption/ionization mass spectrometry; protein identification was made by liquid chromatography. Diverticulitis was used as control (Ctrl). The presence of Hb(n) (n = ?, ?, or hemin)/Hb was validated by Western blotting and immunohistochemistry. We tested for DNA damage (DNAD) by exposing normal colonic epithelial cell line, NCM460, to 10 ?M and 100 ?M of Hb(n)/Hb, individually for 2, 6, and 12 hours. Quantification of Hb-? staining was done by Nikon Elements Advance Research Analysis software. ROS was measured by the production of 8-OHdG. DNAD was assessed by Comet assay. Colonic tissue homogenate antioxidants Nrf2-, CAT-, SOD-, and GPx-expressions were analyzed densitometrically/normalized by ?-actin. RESULTS: Immunohistochemistry of CC/UC mucosal/submucosal compartments stained strongly positive for Hb-? and significantly higher versus Ctrl. NCM460 exposed to Hb(n)/Hb exhibited steadily increasing ROS and subsequent DNAD. DNAD was higher in 10 ?M than 100 ?M in Hb-?/hemin the first 2 hours then plateaued followed by DNAD repair. This may be likely due to apoptosis in the later concentration. Nrf2 enzyme activities among UC, CC, and ulcerative colitis-associated colon cancer (UCAC) were observed impaired in all inflammatory bowel disease subjects. Decreased levels of Nrf2 among patients with UC versus patients with CC with active disease were insignificant as well as versus Ctrls but significantly lower in UCAC versus Ctrl. SOD was decreased in UC and UCAC and GPx in CC but statistically not significant. Comparing CC versus UC, SOD was significantly lower in CC (P < 0.05). CAT was observed increased among patients with CC/UC/UCAC and GPx in UC and UCAC versus Ctrl, respectively, and significantly increased in CC versus Ctrl (P < 0.01). CONCLUSIONS: In the colitides, mucosal/submucosal tissue microenvironments demonstrated pool of free Hb-? chain. In vitro exposure of NCM460 cells to Hb(n)/Hb induced ROS and DNAD. Toxic effect of free Hb-?, in colonic epithelial cells, is therefore through production of ROS formation modulated by impairment of antioxidant effects. Targeting reduction-oxidation-sensitive pathways and transcription factors may offer options for inflammatory bowel disease-management and colitis-related cancer prevention. Twit Text FOAVip Implications of the colonic deposition of free hemoglobin- chain: a previously unknown tissue by-product in inflammatory bowel disease ????Inflamm Bowel Dis http://www.kidney.de/pget.php?&tw=1&pmid=25078150 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25078150 #FOAvip English Wikipedia <ref>{{Cite pmid|25078150 }}</ref> Implications of the colonic deposition of free hemoglobin-? chain: a previously unknown tissue by-product in inflammatory bowel disease #MMPMID25078150 Myers JN ; Schäffer MW ; Korolkova OY ; Williams AD ; Gangula PR ; M'Koma AE Inflamm Bowel Dis 2014[Sep]; 20 (9 ): 1530-47 PMID25078150 show ga BACKGROUND: We analyzed inflamed mucosal/submucosal layers of ulcerative colitis (UC = 63) and Crohn's colitis (CC = 50), and unexpectedly, we unveiled a pool of free hemoglobin alpha (Hb-?) chain. Patients with colitides have increased reactive oxidative stress (ROS), DNA oxidation products, free iron in mucosa, in preneoplastic, and in colitis-cancers and increased risks of developing colorectal cancer. All inflammatory bowel disease-related colorectal cancer lesions are found in segments with colitis. Linking this information, we investigated whether free Hb-? is key transformational stepping that increases colitis-related colorectal cancer vulnerability. METHODS: UC/CC samples were profiled using matrix-assisted laser desorption/ionization mass spectrometry; protein identification was made by liquid chromatography. Diverticulitis was used as control (Ctrl). The presence of Hb(n) (n = ?, ?, or hemin)/Hb was validated by Western blotting and immunohistochemistry. We tested for DNA damage (DNAD) by exposing normal colonic epithelial cell line, NCM460, to 10 ?M and 100 ?M of Hb(n)/Hb, individually for 2, 6, and 12 hours. Quantification of Hb-? staining was done by Nikon Elements Advance Research Analysis software. ROS was measured by the production of 8-OHdG. DNAD was assessed by Comet assay. Colonic tissue homogenate antioxidants Nrf2-, CAT-, SOD-, and GPx-expressions were analyzed densitometrically/normalized by ?-actin. RESULTS: Immunohistochemistry of CC/UC mucosal/submucosal compartments stained strongly positive for Hb-? and significantly higher versus Ctrl. NCM460 exposed to Hb(n)/Hb exhibited steadily increasing ROS and subsequent DNAD. DNAD was higher in 10 ?M than 100 ?M in Hb-?/hemin the first 2 hours then plateaued followed by DNAD repair. This may be likely due to apoptosis in the later concentration. Nrf2 enzyme activities among UC, CC, and ulcerative colitis-associated colon cancer (UCAC) were observed impaired in all inflammatory bowel disease subjects. Decreased levels of Nrf2 among patients with UC versus patients with CC with active disease were insignificant as well as versus Ctrls but significantly lower in UCAC versus Ctrl. SOD was decreased in UC and UCAC and GPx in CC but statistically not significant. Comparing CC versus UC, SOD was significantly lower in CC (P < 0.05). CAT was observed increased among patients with CC/UC/UCAC and GPx in UC and UCAC versus Ctrl, respectively, and significantly increased in CC versus Ctrl (P < 0.01). CONCLUSIONS: In the colitides, mucosal/submucosal tissue microenvironments demonstrated pool of free Hb-? chain. In vitro exposure of NCM460 cells to Hb(n)/Hb induced ROS and DNAD. Toxic effect of free Hb-?, in colonic epithelial cells, is therefore through production of ROS formation modulated by impairment of antioxidant effects. Targeting reduction-oxidation-sensitive pathways and transcription factors may offer options for inflammatory bowel disease-management and colitis-related cancer prevention. |Adolescent [MESH] |Adult [MESH] |Aged [MESH] |Aged, 80 and over [MESH] |Antioxidants/metabolism [MESH] |Blotting, Western [MESH] |Cell Survival [MESH] |Colitis, Ulcerative/complications/metabolism/*pathology [MESH] |Colon/metabolism/*pathology [MESH] |Colonic Neoplasms/metabolism/*pathology [MESH] |Comet Assay [MESH] |Crohn Disease/complications/metabolism/*pathology [MESH] |DNA Damage [MESH] |Female [MESH] |Follow-Up Studies [MESH] |Humans [MESH] |Male [MESH] |Middle Aged [MESH] |NF-E2-Related Factor 2/metabolism [MESH] |Oxidative Stress [MESH] |Prognosis [MESH] |Reactive Oxygen Species/metabolism [MESH] |Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization [MESH] |Young Adult [MESH]Implications of the colonic deposition of free hemoglobin-? chain: a p(epmc).pdf DeepDyve Pubget Overpricing