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10.1016/j.cellsig.2014.05.018 http://scihub22266oqcxt.onion/10.1016/j.cellsig.2014.05.018 C4130781!4130781!24905473     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Signal 2014 ; 26 (10): 2276-83 Nephropedia Template TP {{tp|p=24905473|t=2014. Epidermal Growth Factor Inhibits Transforming Growth Factor-?-Induced Fibrogenic Differentiation Marker Expression through ERK Activation |pdf=|usr=}}{{24905473}} gab.com Text Epidermal Growth Factor Inhibits Transforming Growth Factor- -Induced Fibrogenic Differentiation Marker Expression through ERK Activation JO: Cell Signal http://www.kidney.de/pget.php?&tw=1&pmid=24905473 #FOAvip Transforming growth factor-? (TGF-?) signaling plays an important and complex role in renal fibrogenesis. The seemingly simple TGF-?/Smad cascade is intensively regulated at several levels, including crosstalk with other signaling pathways. Epidermal growth factor (EGF) is a potent mitogen for epithelial cells and is elevated in diseased kidneys. In this study, we examined its effect on TGF-?-induced fibrotic changes in human proximal tubular epithelial cells. Simultaneous treatment with EGF specifically inhibited basal and yTGF-?-induced type-I collagen and ?-smooth muscle actin (?SMA) expression at both mRNA and protein levels. These effects were prevented by inhibition of either the EGF receptor kinase or its downstream MEK kinase but not by blockade of either the JNK or PI3K pathway. Overexpression of a constitutively active MEK1 construct mimicked the inhibitory effect of EGF. Further, EGF suppressed Smad transcriptional activities, as shown by reduced activation of ARE-luc and SBE-luc. Both reductions were prevented by MEK inhibition. However, EGF did not block Smad2 or Smad3 phosphorylation by TGF-?, or Smad2/3 nuclear import. Finally EGF induced the phosphorylation and expression of TGIF, a known TGF-?/Smad repressor. Both the phosphorylation and the induction were blocked by a MEK inhibitor. Overexpression of TGIF abolished TGF-?-induced ?SMA promoter activity. Together these results suggest that EGF inhibits two TGF-?-stimulated markers of EMT through EGF receptor tyrosine kinase and downstream ERK activation, but not through PI3K or JNK. The inhibition results from effector mechanisms downstream of Smads, and most likely involves the transcriptional repressor, TGIF. Twit Text FOAVip Epidermal Growth Factor Inhibits Transforming Growth Factor- -Induced Fibrogenic Differentiation Marker Expression through ERK Activation ????Cell Signal http://www.kidney.de/pget.php?&tw=1&pmid=24905473 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24905473 #FOAvip English Wikipedia <ref>{{Cite pmid|24905473}}</ref> Epidermal Growth Factor Inhibits Transforming Growth Factor-?-Induced Fibrogenic Differentiation Marker Expression through ERK Activation #MMPMID24905473 Liu X; Hubchak SC; Browne JA; Schnaper HW Cell Signal 2014[Oct]; 26 (10): 2276-83 PMID24905473show ga Transforming growth factor-? (TGF-?) signaling plays an important and complex role in renal fibrogenesis. The seemingly simple TGF-?/Smad cascade is intensively regulated at several levels, including crosstalk with other signaling pathways. Epidermal growth factor (EGF) is a potent mitogen for epithelial cells and is elevated in diseased kidneys. In this study, we examined its effect on TGF-?-induced fibrotic changes in human proximal tubular epithelial cells. Simultaneous treatment with EGF specifically inhibited basal and yTGF-?-induced type-I collagen and ?-smooth muscle actin (?SMA) expression at both mRNA and protein levels. These effects were prevented by inhibition of either the EGF receptor kinase or its downstream MEK kinase but not by blockade of either the JNK or PI3K pathway. Overexpression of a constitutively active MEK1 construct mimicked the inhibitory effect of EGF. Further, EGF suppressed Smad transcriptional activities, as shown by reduced activation of ARE-luc and SBE-luc. Both reductions were prevented by MEK inhibition. However, EGF did not block Smad2 or Smad3 phosphorylation by TGF-?, or Smad2/3 nuclear import. Finally EGF induced the phosphorylation and expression of TGIF, a known TGF-?/Smad repressor. Both the phosphorylation and the induction were blocked by a MEK inhibitor. Overexpression of TGIF abolished TGF-?-induced ?SMA promoter activity. Together these results suggest that EGF inhibits two TGF-?-stimulated markers of EMT through EGF receptor tyrosine kinase and downstream ERK activation, but not through PI3K or JNK. The inhibition results from effector mechanisms downstream of Smads, and most likely involves the transcriptional repressor, TGIF. äEpidermal Growth Factor Inhibits Transforming Growth Factor-?-Induced (epmc).pdf DeepDyve Pubget Overpricing