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10.1002/cbic.201402010 http://scihub22266oqcxt.onion/10.1002/cbic.201402010 C4130483!4130483!24828915     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Chembiochem 2014 ; 15 (9): 1317-24 Nephropedia Template TP {{tp|p=24828915|t=2014. Fluorescent Visualization of Src Using Dasatinib-BODIPY |pdf=|usr=}}{{24828915}} gab.com Text Fluorescent Visualization of Src Using Dasatinib-BODIPY JO: Chembiochem http://www.kidney.de/pget.php?&tw=1&pmid=24828915 #FOAvip Many biological experiments are not compatible with the use of immunofluorescence or genetically-encoded fluorescent tags or FRET-based reporters. Conjugation of existing kinase inhibitors to cell-permeable fluorophores can provide a generalized approach to develop fluorescent probes of intracellular kinases. Here, we report the development of a small molecule probe of Src through conjugation of BODIPY to two well-established, dual Src-Abl kinase inhibitors, dasatinib and saracatinib. We show that this approach is not successful for saracatinib, but that largely dasatinib-BODIPY retains the biological activity of its parent compound and can be used to monitor the presence of Src kinase in individual cells by flow cytometry and to track the localization of Src by fixed and live-cell fluorescence microscopy. This strategy may enable generation of additional kinase-specific probes useful in systems not amenable to genetic manipulation or used together with fluorescent proteins to enable a multiplexed assay read-out. Twit Text FOAVip Fluorescent Visualization of Src Using Dasatinib-BODIPY ????Chembiochem http://www.kidney.de/pget.php?&tw=1&pmid=24828915 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24828915 #FOAvip English Wikipedia <ref>{{Cite pmid|24828915}}</ref> Fluorescent Visualization of Src Using Dasatinib-BODIPY #MMPMID24828915 Vetter ML; Zhang Z; Liu S; Wang J; Cho H; Zhang J; Zhang W; Gray NS; Yang PL Chembiochem 2014[Jun]; 15 (9): 1317-24 PMID24828915show ga Many biological experiments are not compatible with the use of immunofluorescence or genetically-encoded fluorescent tags or FRET-based reporters. Conjugation of existing kinase inhibitors to cell-permeable fluorophores can provide a generalized approach to develop fluorescent probes of intracellular kinases. Here, we report the development of a small molecule probe of Src through conjugation of BODIPY to two well-established, dual Src-Abl kinase inhibitors, dasatinib and saracatinib. We show that this approach is not successful for saracatinib, but that largely dasatinib-BODIPY retains the biological activity of its parent compound and can be used to monitor the presence of Src kinase in individual cells by flow cytometry and to track the localization of Src by fixed and live-cell fluorescence microscopy. This strategy may enable generation of additional kinase-specific probes useful in systems not amenable to genetic manipulation or used together with fluorescent proteins to enable a multiplexed assay read-out. äFluorescent Visualization of Src Using Dasatinib-BODIPY (epmc).pdf DeepDyve Pubget Overpricing