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10.1007/s10529-014-1555-9 http://scihub22266oqcxt.onion/10.1007/s10529-014-1555-9 C4127420!4127420 !24930095     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24930095 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biotechnol+Lett 2014 ; 36 (9 ): 1863-8 Nephropedia Template TP {{tp|p=24930095 |t=2014. Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation |pdf=|usr=}}{{24930095 }} gab.com Text Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation JO: Biotechnol Lett http://www.kidney.de/pget.php?&tw=1&pmid=24930095 #FOAvip We report a novel, modular approach to immuno-detection based on antibody recognition and PCR read-out that employs antibody-conjugated bacteriophage and easily-manipulated non-pathogenic viruses as affinity agents. Our platform employs phage genetically tagged for in vivo biotinylation during phage maturation that can easily be linked, through avidin, to any biotinylated affinity agent, including full-length antibodies, peptides, lectins or aptamers. The presence of analyte is reported with high sensitivity through real-time PCR. This approach avoids the need to clone antibody-encoding DNA fragments, allows the use of full-length, high affinity antibodies and, by having DNA reporters naturally encapsulated inside the bacteriophage, greatly reduces nonspecific binding of DNA. We validate the efficacy of this new approach through the detection of Vascular Endothelial Growth Factor, a known angiogenic cancer biomarker protein, at attomolar concentrations in bronchoalveolar lavage fluid. Twit Text FOAVip Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation ????Biotechnol Lett http://www.kidney.de/pget.php?&tw=1&pmid=24930095 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24930095 #FOAvip English Wikipedia <ref>{{Cite pmid|24930095 }}</ref> Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation #MMPMID24930095 Litvinov J ; Hagström AE ; Lopez Y ; Adhikari M ; Kourentzi K ; Strych U ; Monzon FA ; Foster W ; Cagle PT ; Willson RC Biotechnol Lett 2014[Sep]; 36 (9 ): 1863-8 PMID24930095 show ga We report a novel, modular approach to immuno-detection based on antibody recognition and PCR read-out that employs antibody-conjugated bacteriophage and easily-manipulated non-pathogenic viruses as affinity agents. Our platform employs phage genetically tagged for in vivo biotinylation during phage maturation that can easily be linked, through avidin, to any biotinylated affinity agent, including full-length antibodies, peptides, lectins or aptamers. The presence of analyte is reported with high sensitivity through real-time PCR. This approach avoids the need to clone antibody-encoding DNA fragments, allows the use of full-length, high affinity antibodies and, by having DNA reporters naturally encapsulated inside the bacteriophage, greatly reduces nonspecific binding of DNA. We validate the efficacy of this new approach through the detection of Vascular Endothelial Growth Factor, a known angiogenic cancer biomarker protein, at attomolar concentrations in bronchoalveolar lavage fluid. |*Biotinylation [MESH] |*Nanoparticles [MESH] |Antibodies, Viral/metabolism [MESH] |Bacteriophages/*genetics [MESH] |Humans [MESH] |Immunoassay/methods [MESH] |Lectins/metabolism [MESH] |Peptides/metabolism [MESH] |Protein Binding [MESH] |Real-Time Polymerase Chain Reaction/*methods [MESH] |Sensitivity and Specificity [MESH]Ultrasensitive immuno-detection using viral nanoparticles with modular(epmc).pdf DeepDyve Pubget Overpricing