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10.1097/WNR.0000000000000211

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C4126080!4126080!25004078
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suck abstract from ncbi


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pmid25004078      Neuroreport 2014 ; 25 (13): 1018-23
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  • Multi-Day Administration of Ivermectin is Effective in Reducing Alcohol Intake in Mice at Doses Shown to be Safe in Humans #MMPMID25004078
  • Yardley MM; Neely M; Huynh N; Asatryan L; Louie SG; Alkana RL; Davies DL
  • Neuroreport 2014[Sep]; 25 (13): 1018-23 PMID25004078show ga
  • Ivermectin (IVM), an FDA approved anthelmintic agent, can significantly reduce ethanol intake in mice following acute administration. The current study evaluates the sustainability and safety of multi-day IVM administration in reducing 10E intake in mice at a dose shown to be safe in humans. We tested the effect of 10-day administration of IVM (3.0 mg/kg/day; i.p.) on reducing 10% v/v alcohol (10E) intake in C57BL/6J mice using a 24-h, two-bottle choice paradigm. On the 10th day of IVM administration, mice were sacrificed at 0, 0.5, 2, 8, 32, 48 and 72 hours post-injection. Brain tissue and plasma samples were collected and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Analysis of variance (ANOVA) was used to assess the effect of 10-day IVM administration on 10E intake, 10E preference, water intake and total fluid intake with Dunnett?s Multiple Comparison post-hoc test. Individual student?s t-tests were also used to further quantify changes in these dependent variables. IVM significantly decreased 10E intake over a 9-day period (p<0.01). Pre IVM 10E intake was 9.1 ± 3.2 g/kg/24-h. Following the 9th day of IVM injections, intake dropped by almost 30% (p<0.05). IVM had no effect on total water intake or mouse weight throughout the study; however, there was a significant decrease in both preference for 10E (p<0.01) and total fluid intake (p<0.05). Multi-day administration of IVM significantly reduces 10E intake and preference in animals without causing any apparent adverse effects at a dose shown to be safe in humans.
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