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10.1016/j.cbpa.2014.05.010 http://scihub22266oqcxt.onion/10.1016/j.cbpa.2014.05.010 C4123530!4123530!24956235     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Opin+Chem+Biol 2014 ; ä (ä): 103-11 Nephropedia Template TP {{tp|p=24956235|t=2014. The bright future of single-molecule fluorescence imaging |pdf=|usr=}}{{24956235}} gab.com Text The bright future of single-molecule fluorescence imaging JO: Curr Opin Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=24956235 #FOAvip Single-molecule Förster resonance energy transfer (smFRET) is an essential and maturing tool to probe biomolecular interactions and conformational dynamics in vitro and, increasingly, in living cells. Multi-color smFRET enables the correlation of multiple such events and the precise dissection of their order and timing. However, the requirements for good spectral separation, high time resolution, and extended observation times place extraordinary demands on the fluorescent labels used in such experiments. Together with advanced experimental designs and data analysis, the development of long-lasting, non-fluctuating fluorophores is therefore proving key to progress in the field. Recently developed strategies for obtaining ultra-stable organic fluorophores spanning the visible spectrum are underway that will enable multi-color smFRET studies to deliver on their promise of previously unachievable biological insights. Twit Text FOAVip The bright future of single-molecule fluorescence imaging ????Curr Opin Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=24956235 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24956235 #FOAvip English Wikipedia <ref>{{Cite pmid|24956235}}</ref> The bright future of single-molecule fluorescence imaging #MMPMID24956235 Juette MF; Terry DS; Wasserman MR; Zhou Z; Altman RB; Zheng Q; Blanchard SC Curr Opin Chem Biol 2014[Jun]; ä (ä): 103-11 PMID24956235show ga Single-molecule Förster resonance energy transfer (smFRET) is an essential and maturing tool to probe biomolecular interactions and conformational dynamics in vitro and, increasingly, in living cells. Multi-color smFRET enables the correlation of multiple such events and the precise dissection of their order and timing. However, the requirements for good spectral separation, high time resolution, and extended observation times place extraordinary demands on the fluorescent labels used in such experiments. Together with advanced experimental designs and data analysis, the development of long-lasting, non-fluctuating fluorophores is therefore proving key to progress in the field. Recently developed strategies for obtaining ultra-stable organic fluorophores spanning the visible spectrum are underway that will enable multi-color smFRET studies to deliver on their promise of previously unachievable biological insights. äThe bright future of single-molecule fluorescence imaging (epmc).pdf DeepDyve Pubget Overpricing